CAJ | 학술논문

目的：研究CC趋化因子拮抗剂Met-RANTES对rd小鼠视网膜外核层形态的影响，探讨其在rd小鼠遗传性视网膜变性中的潜在治疗作用。方法以出生后7drd小鼠作为研究对象，随机分为5组，分别给予腹腔注射Met-RANTES、PBS和玻璃体腔注射不同浓度Met-RANTES、PBS，直到rd小鼠出生后14d，过量麻醉法处死后摘取眼球制作冰冻切片，HE染色后观察测量视网膜后极部外核层厚度。结果腹腔注射met-RANTES、玻璃体腔注射0．7μlMet-RANTESrd小鼠与对照小鼠视网膜外核层细胞层数无明显变化（P＞0．05）。玻璃体腔注射1．0μlMet-RANTES和1．5μlMet-RANTES眼球视网膜外核层比对照眼球视网膜外核层厚，有统计学差异（P＜0．05）。结论CC趋化因子拮抗剂Met-RANTES对rd小鼠遗传性视网膜变性可能存有潜在治疗作用。
목적：연구CC추화인자길항제Met-RANTES대rd소서시망막외핵층형태적영향，탐토기재rd소서유전성시망막변성중적잠재치료작용。방법이출생후7drd소서작위연구대상，수궤분위5조，분별급여복강주사Met-RANTES、PBS화파리체강주사불동농도Met-RANTES、PBS，직도rd소서출생후14d，과량마취법처사후적취안구제작빙동절편，HE염색후관찰측량시망막후겁부외핵층후도。결과복강주사met-RANTES、파리체강주사0．7μlMet-RANTESrd소서여대조소서시망막외핵층세포층수무명현변화（P＞0．05）。파리체강주사1．0μlMet-RANTES화1．5μlMet-RANTES안구시망막외핵층비대조안구시망막외핵층후，유통계학차이（P＜0．05）。결론CC추화인자길항제Met-RANTES대rd소서유전성시망막변성가능존유잠재치료작용。
Objective To study the effect of chemokine antagonist Met-RANTES on outer nuclear layer reti-na in the retinal degenerative process in rd mice and further explore its role in the photoreceptor degeneration .Methods Rd mice at postnatal day ( P) 7 were enrolled and divided to 5 groups.The subjects were treated with intraperitoneal injection or intravitreous injection with Met-RANTES,PBS in different concentrations .The mice were sacrificed and reti-nal sections were prepared ,which were stained by hematoxylin-eosin staining ( HE) .Then the thickness of outer nuclear layer retinas were detected the thickness of outer nuclear layer retina respectively .The use and care of the animals com-plied with the guideline of ARVO .Results There was no evident diffiernce between the rd mice which were treated by Met-RANTES and their control group(P>0.05).So did the rd mice which were treated by 0.7μl Met-RANTES with glass body cavity injection .However,the rd mice which treated by 1.0μl or 1.5μl Met-RANTES were obviously thicker than their control retinas ( P<0 .05 ) .Conclusion Chemokine antagonist Met-RANTES may poly a therapeutic role in the retinal degenerative process in rd mice .