CAJ | 학술논문

目的：观察胸腺五肽（ TP-5）对重度创伤失血性休克大鼠免疫功能的影响。方法54只健康雄性SD大鼠随机分为3组，即对照组（ n ＝6）、重度创伤失血性休克模型组（模型组， n ＝24）、TP-5复苏组（TP-5组， n ＝24），在复苏后12 h（ T1）、24 h（ T2）、48 h（ T3）、72 h（ T4）各时间窗抽取静脉血，应用流式细胞仪测量T淋巴细胞亚群含量，并应用ELISA法测量肠道黏液内分泌性免疫球蛋白A（ SIgA）含量。结果模型组与TP-5组病死率均为41．17％（1/24），差异无统计学意义（ P ＞0．05）。与对照组比较，模型组T1～4的CD4＋、CD4＋/CD8＋、SIgA含量均明显下降，CD8＋明显升高（ P ＜0．01， P ＜0．05）；与模型组比较，TP-5组随复苏时间延长，各项指标逐渐恢复，差异有统计学意义（ P ＜0．01， P ＜0．05）。结论早期联合应用TP-5复苏治疗对重度创伤失血性休克大鼠免疫功能有一定保护作用。
목적：관찰흉선오태（ TP-5）대중도창상실혈성휴극대서면역공능적영향。방법54지건강웅성SD대서수궤분위3조，즉대조조（ n ＝6）、중도창상실혈성휴극모형조（모형조， n ＝24）、TP-5복소조（TP-5조， n ＝24），재복소후12 h（ T1）、24 h（ T2）、48 h（ T3）、72 h（ T4）각시간창추취정맥혈，응용류식세포의측량T림파세포아군함량，병응용ELISA법측량장도점액내분비성면역구단백A（ SIgA）함량。결과모형조여TP-5조병사솔균위41．17％（1/24），차이무통계학의의（ P ＞0．05）。여대조조비교，모형조T1～4적CD4＋、CD4＋/CD8＋、SIgA함량균명현하강，CD8＋명현승고（ P ＜0．01， P ＜0．05）；여모형조비교，TP-5조수복소시간연장，각항지표축점회복，차이유통계학의의（ P ＜0．01， P ＜0．05）。결론조기연합응용TP-5복소치료대중도창상실혈성휴극대서면역공능유일정보호작용。
Objective To investigate the influence of TP-5 on immune function of rats with severe traumatic hemor-rhagic shock.Methods 54 healthy male SD rats were randomly divided into three groups , the control group( n =6), severe traumatic hemorrhagic shock model (model group, n =24), TP-5 resuscitation group(TP-5 group, n =24), in recovery after 12 ( T1 ) , 24 ( T2 ) , 48 ( T3 ) , 72 ( T4 ) each time window picked up venous blood , used flow cytometry to measure the con-tent of T lymphocyte subsets and used ELISA to measure SIgA content within intestinal mucus .Results Model group and TP-5 group's mortality rate were 4.17%(1/24), the difference was not statistically significant ( P >0.05).Compared with the control group, model group T1-4 of CD4+,CD4+/CD8+,SIgA levels were significantly decreased ,CD8+was significantly higher (P <0.01, P <0.05); compared with the model group, TP-5 group with prolonged recovery, the index gradually recov-ered, significantly better than the model group ( P <0.01, P <0.05).Conclusion Combined TP-5 early resuscitation of severe traumatic hemorrhagic shock in rats immune function have a protective effect .