滨州医学院学报
濱州醫學院學報
빈주의학원학보
JOURNAL OF BINZHOU MEDICAL COLLEGE
2014年
2期
84-86
,共3页
孙若思%梁惠%孙藜玮%王林林
孫若思%樑惠%孫藜瑋%王林林
손약사%량혜%손려위%왕림림
表没食子儿茶素没食子酸酯%H22肝癌%丙二醛%超氧化物歧化酶%谷胱甘肽过氧化物酶
錶沒食子兒茶素沒食子痠酯%H22肝癌%丙二醛%超氧化物歧化酶%穀胱甘肽過氧化物酶
표몰식자인다소몰식자산지%H22간암%병이철%초양화물기화매%곡광감태과양화물매
Epigallocatechin gallate%H22 tumor%Malonyl dialehyde%Superoxide dismutase%Glutathione peroxidase
目的:研究EGCG对小鼠 H22肝癌生长及抗氧化作用的影响。方法给予荷瘤鼠喂饲20、40、80 mg · kg -1· d-1 EGCG ,阳性对照组小鼠腹腔注射20 mg · kg -1· d-15-氟尿嘧啶(5-Fu),并设空白及模型对照组,2周后处死动物,无菌条件下取肿瘤、脾和胸腺,称重,计算抑瘤率及脏器指数。以试剂盒测定各组小鼠血浆中丙二醛(MDA )含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力,进行统计学分析。结果 EGCG各剂量组小鼠 H22肿瘤生长速度较模型组缓慢(P<0.05),在一定范围内与剂量呈正相关,低、中、高三剂量组的抑瘤率分别为25.572%、35.367%、44.52%;EGCG各剂量组的胸腺指数上升,与模型组及5-Fu对照组均有显著性差异( P<0.05);EGCG各剂量组的脾指数上升,与模型组之间有显著性差异(P<0.05),中、高剂量EGCG组与5-Fu组之间脾指数有显著性差异(P<0.05);与模型组比较,EGCG各剂量组及5-Fu组对照组小鼠血浆中MDA的含量明显降低,SOD、GSH-Px活力明显升高,差异有统计学意义(P<0.05)。结论EGCG各剂量组能较好的抑制 H22小鼠肿瘤增长,具有较高的体内抑瘤活性;EGCG具有良好的抗氧化作用。
目的:研究EGCG對小鼠 H22肝癌生長及抗氧化作用的影響。方法給予荷瘤鼠餵飼20、40、80 mg · kg -1· d-1 EGCG ,暘性對照組小鼠腹腔註射20 mg · kg -1· d-15-氟尿嘧啶(5-Fu),併設空白及模型對照組,2週後處死動物,無菌條件下取腫瘤、脾和胸腺,稱重,計算抑瘤率及髒器指數。以試劑盒測定各組小鼠血漿中丙二醛(MDA )含量及超氧化物歧化酶(SOD)和穀胱甘肽過氧化物酶(GSH-Px)的活力,進行統計學分析。結果 EGCG各劑量組小鼠 H22腫瘤生長速度較模型組緩慢(P<0.05),在一定範圍內與劑量呈正相關,低、中、高三劑量組的抑瘤率分彆為25.572%、35.367%、44.52%;EGCG各劑量組的胸腺指數上升,與模型組及5-Fu對照組均有顯著性差異( P<0.05);EGCG各劑量組的脾指數上升,與模型組之間有顯著性差異(P<0.05),中、高劑量EGCG組與5-Fu組之間脾指數有顯著性差異(P<0.05);與模型組比較,EGCG各劑量組及5-Fu組對照組小鼠血漿中MDA的含量明顯降低,SOD、GSH-Px活力明顯升高,差異有統計學意義(P<0.05)。結論EGCG各劑量組能較好的抑製 H22小鼠腫瘤增長,具有較高的體內抑瘤活性;EGCG具有良好的抗氧化作用。
목적:연구EGCG대소서 H22간암생장급항양화작용적영향。방법급여하류서위사20、40、80 mg · kg -1· d-1 EGCG ,양성대조조소서복강주사20 mg · kg -1· d-15-불뇨밀정(5-Fu),병설공백급모형대조조,2주후처사동물,무균조건하취종류、비화흉선,칭중,계산억류솔급장기지수。이시제합측정각조소서혈장중병이철(MDA )함량급초양화물기화매(SOD)화곡광감태과양화물매(GSH-Px)적활력,진행통계학분석。결과 EGCG각제량조소서 H22종류생장속도교모형조완만(P<0.05),재일정범위내여제량정정상관,저、중、고삼제량조적억류솔분별위25.572%、35.367%、44.52%;EGCG각제량조적흉선지수상승,여모형조급5-Fu대조조균유현저성차이( P<0.05);EGCG각제량조적비지수상승,여모형조지간유현저성차이(P<0.05),중、고제량EGCG조여5-Fu조지간비지수유현저성차이(P<0.05);여모형조비교,EGCG각제량조급5-Fu조대조조소서혈장중MDA적함량명현강저,SOD、GSH-Px활력명현승고,차이유통계학의의(P<0.05)。결론EGCG각제량조능교호적억제 H22소서종류증장,구유교고적체내억류활성;EGCG구유량호적항양화작용。
Objective To investigate the antitumor and antioxidant effects of different dosages of epigallocatechin-gallate (EGCG) on mice .Methods H22 tumor bearing mice were injected with different doses of EGCG (20 ,40 ,80mg · kg -1 d-1 re-spectively) ,and the control group of mice were administrated with 20mg · kg -1 · d-1 5-Fluorouracil (5-Fu) .All the mice were killed 2 week after tumor inoculated .Tumors ,spleens and thymus were removed ,then tumor inhibition rates and viscera index were calculated .The concentration of superoxide dismutase (SOD) ,malonyl dialehyde (MDA) and glutathione peroxidase (GSH-Px)in blood plasma were also detected with the test kit .Results Tumor weight in EGCG groups was increased slower than the model group ,and the inhibition rate was raised in accordance with the dosages of EGCG .The inhibition rates of low-, middle-,high-dose EGCG groups were 25.572% ,35.367% and 44.52% respectively .Furthermore ,the viscera index of the thymus in EGCG groups had risen and had dramatic difference from the model group and 5-Fu group(P<0.05) .The viscera in-dex of the spleens in EGCG groups had risen and had dramatic difference from the model group (P< 0.05) .Moreover ,the difference between middle-,high-dose EGCG groups and the 5-Fu group was dramatic (P< 0.05) .Compared with control group ,GSH-Px and SOD activity tend to increase and MDA content was a downward trend in different EGCG groups (P<0.05) .Conclusion Different dosages of EGCG can obviously inhibit the H22 tumor growth and displays a high anti-tumor activ-ity ;EGCG could improve the body's antioxidant levels .