CAJ | 학술논문

目的：研究外源ATP对Treg细胞特异性及其表型的影响。方法：制备胸腺单细胞悬液，分别加入0、0.01、0.1、0.5和1 mmol/L浓度的外源性ATP，作用24 h后利用荧光检测法分析胞外ATP浓度，然后利用流式细胞仪分析作用72 h后胸腺细胞中Treg细胞比例的变化及其FOXP3和CD39表达的变化。结果：ATP作用72 h后，与对照组比较，胸腺细胞数减少，Treg细胞比例下调，以1 mmol/L作用组下调显著(t=5.260，=0.034)；Treg细胞中FOXP3和CD39的平均荧光强度(MFI)上调，以较高浓度(0.5和1 mmol/L)ATP作用组上调显著(P均<0.05)。结论：较高浓度ATP会导致Treg细胞数减少，但存活Treg细胞特异性和功能相关表型因子表达增强。P
목적：연구외원ATP대Treg세포특이성급기표형적영향。방법：제비흉선단세포현액，분별가입0、0.01、0.1、0.5화1 mmol/L농도적외원성ATP，작용24 h후이용형광검측법분석포외ATP농도，연후이용류식세포의분석작용72 h후흉선세포중Treg세포비례적변화급기FOXP3화CD39표체적변화。결과：ATP작용72 h후，여대조조비교，흉선세포수감소，Treg세포비례하조，이1 mmol/L작용조하조현저(t=5.260，=0.034)；Treg세포중FOXP3화CD39적평균형광강도(MFI)상조，이교고농도(0.5화1 mmol/L)ATP작용조상조현저(P균<0.05)。결론：교고농도ATP회도치Treg세포수감소，단존활Treg세포특이성화공능상관표형인자표체증강。P
OBJECTIVE: To analyze the effect of exogenous ATP exposure on the expression of phenotype factors in thymic regulatory T cells(Treg). METHODS:Thymocytes were separated and exposed to 0,0.01,0.1,0.5, 1 mmol/L of exogenous ATP in vitro,then at 72 h the percentage of Treg in thymocytes and their expressions of FOXP3,CD39 were analyzed using FACS. RESULTS:Both the number of thymocytes and the percentage of its Treg subset decreased,particularly in 1 mmol/L ATP exposure groups(t=5.260,P=0.034) and the mean fluorescence intensity of both FOXP3 and CD39 increased in the higher dosage ATP exposure group(0.5 and 1 mmol/L).CONCLUSION:Treg cell numbed decreased but the specificity and function-associated phenotypes of the viable Treg were enhanced after exposure to higher concentration of ATP.