癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
2期
113-116
,共4页
精氨酸酶-1%原发性肝癌%肝转移癌%细针穿刺活检
精氨痠酶-1%原髮性肝癌%肝轉移癌%細針穿刺活檢
정안산매-1%원발성간암%간전이암%세침천자활검
arginase-1%primary hepatic carcinoma%hepatic metastasis%fine-needle aspiration biopsy
目的：探讨精氨酸酶-1(Arg-1)在原发性肝癌(PHC)和肝转移癌(HM)鉴别中的意义。方法：123例肝癌患者行超声引导下细针穿刺活检，标本制作成细胞块进行免疫组化染色，观察Arg-1表达情况。所有患者行手术切除后进行组织病理学诊断，结合细胞学、血清肿瘤标记物、临床及影像学随访结果进行最终判定。计算采用Arg-1鉴别PHC和HM的敏感度和特异性。结果：123例患者中PHC 78例，HM 45例。在PHC患者中，Arg-1呈高表达(83.3%)且以强染色为主(60.3%)，高、中度分化肝细胞癌患者中阳性表达率(92.9%)高于低分化患者(76.9%)，在胆管细胞型肝癌患者中不表达，在混合型肝癌患者中阳性表达率为75%；在HM患者中，Arg-1阳性表达率较低(8.9%)，存在于结直肠癌、胰腺癌、乳腺癌肝转移患者中且均为弱染色，其他原发部位肝转移患者未见Arg-1阳性表达。Arg-1用于PHC和HM鉴别的敏感度为83.3%，特异性为91.1%。结论：细针穿刺活检标本行Arg-1免疫组化染色用于PHC和HM鉴别的敏感度和特异性均较高，值得进一步推广。
目的：探討精氨痠酶-1(Arg-1)在原髮性肝癌(PHC)和肝轉移癌(HM)鑒彆中的意義。方法：123例肝癌患者行超聲引導下細針穿刺活檢，標本製作成細胞塊進行免疫組化染色，觀察Arg-1錶達情況。所有患者行手術切除後進行組織病理學診斷，結閤細胞學、血清腫瘤標記物、臨床及影像學隨訪結果進行最終判定。計算採用Arg-1鑒彆PHC和HM的敏感度和特異性。結果：123例患者中PHC 78例，HM 45例。在PHC患者中，Arg-1呈高錶達(83.3%)且以彊染色為主(60.3%)，高、中度分化肝細胞癌患者中暘性錶達率(92.9%)高于低分化患者(76.9%)，在膽管細胞型肝癌患者中不錶達，在混閤型肝癌患者中暘性錶達率為75%；在HM患者中，Arg-1暘性錶達率較低(8.9%)，存在于結直腸癌、胰腺癌、乳腺癌肝轉移患者中且均為弱染色，其他原髮部位肝轉移患者未見Arg-1暘性錶達。Arg-1用于PHC和HM鑒彆的敏感度為83.3%，特異性為91.1%。結論：細針穿刺活檢標本行Arg-1免疫組化染色用于PHC和HM鑒彆的敏感度和特異性均較高，值得進一步推廣。
목적：탐토정안산매-1(Arg-1)재원발성간암(PHC)화간전이암(HM)감별중적의의。방법：123례간암환자행초성인도하세침천자활검，표본제작성세포괴진행면역조화염색，관찰Arg-1표체정황。소유환자행수술절제후진행조직병이학진단，결합세포학、혈청종류표기물、림상급영상학수방결과진행최종판정。계산채용Arg-1감별PHC화HM적민감도화특이성。결과：123례환자중PHC 78례，HM 45례。재PHC환자중，Arg-1정고표체(83.3%)차이강염색위주(60.3%)，고、중도분화간세포암환자중양성표체솔(92.9%)고우저분화환자(76.9%)，재담관세포형간암환자중불표체，재혼합형간암환자중양성표체솔위75%；재HM환자중，Arg-1양성표체솔교저(8.9%)，존재우결직장암、이선암、유선암간전이환자중차균위약염색，기타원발부위간전이환자미견Arg-1양성표체。Arg-1용우PHC화HM감별적민감도위83.3%，특이성위91.1%。결론：세침천자활검표본행Arg-1면역조화염색용우PHC화HM감별적민감도화특이성균교고，치득진일보추엄。
OBJECTIVE: To study the significance of arginase-1 (Arg-1) in identifying primary hepatic carcinoma (PHC) and hepatic metastasis (HM). METHODS:Fine-needle aspiration biopsies (FNABs) were performed in 123 patients,cell block were made and immunohistochemical staining were applied. The expression of arginase-1 was measured. All patients received surgical resection and histopathologic diagnosis were obtained. Cytology,serum tumor markers,clinical and radiographic follow-up results were used for a final decision. The sensitivity and specificity of Arg-1 in identifying PHC and HM were calculated. RESULTS:123 patients diagnosed to have PHC (78 cases) or HM (45 cases). High expression was present in PHC group (83.3%) and stained strongly (60.3%). Positive expression rate of highly and moderately differentiated patients (92.9%) were higher than lowly differentiated patients (76.9%),with no expression in cholangiocarcinoma patients. In the hybrid liver cancer patients the positive expression rate was 75%. In patients with HM,Arg-1 positive expression rate was low (8.9%) with weak staining,and were found in HM from colorectal cancer,pancreatic cancer. HM from other primary sites showed no Arg-1 positive expression. The sensitivity and specificity of Arg-1 in identifying PHC and HM were 83.3% and 91.1%,respectively. CONCLUSION:Arginase-1 may be a more sensitive marker of hepatocellular carcinoma (HCC) and hepatic metastasis. FNABs with Arg-1 immunohistochemical staining was sensitive and specific in identifying PHC and HM and warrant further promotion.
