CAJ | 학술논문

红细胞是维持生命最重要的细胞之一，p H是影响其结构功能的重要因素。结合人血液中存在血清白蛋白，且可能会对红细胞起作用的特点，在模拟人体内血液中血清白蛋白与血红蛋白摩尔比值的条件下，研究pH协同血清白蛋白对单个活态人红细胞的形态，细胞膜变形能力及胞内血红蛋白结构变化的影响。结果显示，不同pH下，红细胞的表面形态、膜变形能力的变化规律与其胞内血红蛋白结构的变化有关：在p H 4.14，4.76，10.18时，红细胞胞内血红蛋白螺旋结构的减少、疏水性氨基酸的暴露，血红素珠蛋白结合状态的改变，影响了血红蛋白结构的稳定性，从而导致红细胞的结构发生变化，其形态、膜变形能力与正常值相比，变化结果显著。而在p H 6.51和7.80下，血红蛋白的拉曼谱结果并未发现有上述变化情况，胞内血红蛋白的结构没有发生改变，红细胞的形态、膜变形能力也接近于正常值。在p H 5.49和8.76下，红细胞的表面形态和膜变形能力都有不同程度的变差，但其胞内血红蛋白的结构并没有改变，提示红细胞的形态和变形能力的改变可能是可逆的。结果表明，p H协同血清白蛋白的作用下，血清白蛋白能对红细胞起保护作用，增强红细胞调节、缓冲环境pH变化的能力。研究结果全面地揭示了在模拟人体内血液中血清白蛋白与血红蛋白摩尔比值的情况下，红细胞结构功能的变化情况，不仅有助于阐明红细胞在有关生理、病理状态下的结构与功能的变化，而且对其临床表现与有关防治对策都有重要的指导意义。
홍세포시유지생명최중요적세포지일，p H시영향기결구공능적중요인소。결합인혈액중존재혈청백단백，차가능회대홍세포기작용적특점，재모의인체내혈액중혈청백단백여혈홍단백마이비치적조건하，연구pH협동혈청백단백대단개활태인홍세포적형태，세포막변형능력급포내혈홍단백결구변화적영향。결과현시，불동pH하，홍세포적표면형태、막변형능력적변화규률여기포내혈홍단백결구적변화유관：재p H 4.14，4.76，10.18시，홍세포포내혈홍단백라선결구적감소、소수성안기산적폭로，혈홍소주단백결합상태적개변，영향료혈홍단백결구적은정성，종이도치홍세포적결구발생변화，기형태、막변형능력여정상치상비，변화결과현저。이재p H 6.51화7.80하，혈홍단백적랍만보결과병미발현유상술변화정황，포내혈홍단백적결구몰유발생개변，홍세포적형태、막변형능력야접근우정상치。재p H 5.49화8.76하，홍세포적표면형태화막변형능력도유불동정도적변차，단기포내혈홍단백적결구병몰유개변，제시홍세포적형태화변형능력적개변가능시가역적。결과표명，p H협동혈청백단백적작용하，혈청백단백능대홍세포기보호작용，증강홍세포조절、완충배경pH변화적능력。연구결과전면지게시료재모의인체내혈액중혈청백단백여혈홍단백마이비치적정황하，홍세포결구공능적변화정황，불부유조우천명홍세포재유관생리、병리상태하적결구여공능적변화，이차대기림상표현여유관방치대책도유중요적지도의의。
In the present work ,a cell environment which mimicked the real body environment according to the concentration ra-dio between serum albumin and hemoglobin was built ,and the cell morphology ,the membrane deformation capacity ,and the structure of intracellular hemoglobin of single human living erythrocyte under the effect of pH and serum albumin were studied . It was found that at different suspension pH ,the magnitude of variations in cell shape and membrane deformation capacity chan-ges with the structural changes of the intracellular hemoglobin .At pH 4.14 ,4.76 and 10.18 ,the loss of helical structure for hemoglobin ,exposing of the hydrophobic amino acid in the globin chains ,and changing of the combination of heme and globin , would completely destroy the stability of hemoglobin’s structure ,which seriously changes RBC’s morphology and membrane de-formation capacity .While at pH 6.51 and 7.80 ,the Raman spectra of erythrocytes are found to have no such changes ,indicating that the structure of intracellular hemoglobin was not varied ,thus the cell morphology and membrane deformation capacity are quite close to the normal values .At pH 5.49 and 8.76 ,RBC’s morphology and membrane deformation capacity have different degrees of variation ,but the structure of intracellular hemoglobin has not changed ,suggesting that the cell morphology and membrane deformation capacity may be reversible .The results suggest that in the suspension solution containing serum albumin , erythrocytes have better ability to regulate and control the variation of the extracellular pH .In summary ,upon building an envi-ronment which contains the same concentration radio of serum albumin to hemoglobin in the blood ,this work performed system-atic studies on the effect of pH on human erythrocytes .It can not only help to solve the problems about the mechanism of the structural and functional changes of erythrocytes induced by environmental pH ,but also elucidates the possible variation of the e-rythrocyte under different pathologic states .It is therefore significant for the prevention and treatment of some diseases .