CAJ | 학술논문

目的：对汉族人颅内动脉瘤与可溶性环氧化物酶2(EPHX2)基因多态性二者之间的关系进行研究。方法选取颅内动脉瘤患者221例为观察组，同期在院就诊的无颅内动脉瘤患者238例为对照组，通过直接聚合酶链反应(PCR)产物测序以及 PCR 对两组患者 EPHX2基因进行检测，并就其 rs2291635、rs751141两个多态性位点各自基因型频率进行对比分析。结果在 EPHX2基因位点 rs751141多态性基因型分布方面，观察组患者中女性 C 等位基因及 CC 基因型频率占总体分别为83.3%(180/216)及71.3%(77/108)，显著性高于对照组75.8%(182/240)及58.3%(70/120)(均 P <0.05)。两组患者 rs751141等位基因基因型频率在不分性别以及男性患者中的分布差异无统计学意义(均 P >0.05)；两组患者 EPHX2基因位点 rs2291635在不分性别、女性、男性中的基因频率同样无统计学意义(均 P >0.05)。结论汉族人群体中女性颅内动脉瘤的发生可能与 rs751141基因位点所具有的异质性存在关联性。
목적：대한족인로내동맥류여가용성배양화물매2(EPHX2)기인다태성이자지간적관계진행연구。방법선취로내동맥류환자221례위관찰조，동기재원취진적무로내동맥류환자238례위대조조，통과직접취합매련반응(PCR)산물측서이급 PCR 대량조환자 EPHX2기인진행검측，병취기 rs2291635、rs751141량개다태성위점각자기인형빈솔진행대비분석。결과재 EPHX2기인위점 rs751141다태성기인형분포방면，관찰조환자중녀성 C 등위기인급 CC 기인형빈솔점총체분별위83.3%(180/216)급71.3%(77/108)，현저성고우대조조75.8%(182/240)급58.3%(70/120)(균 P <0.05)。량조환자 rs751141등위기인기인형빈솔재불분성별이급남성환자중적분포차이무통계학의의(균 P >0.05)；량조환자 EPHX2기인위점 rs2291635재불분성별、녀성、남성중적기인빈솔동양무통계학의의(균 P >0.05)。결론한족인군체중녀성로내동맥류적발생가능여 rs751141기인위점소구유적이질성존재관련성。
Objective To study the relationship of soluble epoxide hydrolase 2 (EPHX2 )gene polymorphism and intracranial aneurysm in Han nationality.Methods A total of 221 intracranial aneurysm patients were included as the subjects of this study(observation group).Another 238 patients without intracranial aneurysm(reference group) diagnosed in Nanfang Hospital,Southern Medical University in the same period were taken.Direct PCR product sequencing and PCR were used to detect the EPHX2 gene of patients in two groups.And contrastive analysis was made for the genotype frequency of rs2291 635 and rs75 1 141 two polymorphic sites.Results As for the rs75 1 141 polymorphic genotype distribution at EPHX2 gene locus,the C allele and CC genotype frequency of female patients in observation group accounted for 83.3%(180/21 6 )and 71.3%(77/108),respectively,which were significantly higher than those of control group,75.8% (182/240),58.3% (70/120)(P < 0.05 ).The distribution of rs75 1 141 genotype frequency among all the patients and male patients of two groups showed no significant difference (all P >0.05).The distribution of rs2291 635 genotype frequency at EPHX2 gene locus among all the patients,female,and male patients showed no significant difference (all P >0.05).Conclusion The occurrence of intracranial aneurysm among females of Han nationality may be associated with the heterogeneity of rs75 1 141 gene locus.