食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
6期
93-95
,共3页
郑丽雪%刘梦滢%王立梅%齐斌
鄭麗雪%劉夢瀅%王立梅%齊斌
정려설%류몽형%왕립매%제빈
谷胱甘肽%酿酒酵母%金属离子
穀胱甘肽%釀酒酵母%金屬離子
곡광감태%양주효모%금속리자
glutathione%Saccharomyces cerevisiae%metal ion
通过摇瓶发酵培养,研究了Cu2+,Mg2+,K+,Fe2+,Mn2+5种金属离子在不同发酵时间添加对酿酒酵母CS10515-1生产谷胱甘肽的影响。实验结果表明:在发酵初期(0 h),当K+、Mg2+、Fe2+添加量分别为0.15、0.12、0.09 g/L时,GSH的产量分别为88.53、52.73、41.42 mg/L,相比空白对照分别提高了63.50%、36.58%和44.25%。在15 h时,当Mg2+添加量为0.09 g/L时,GSH产量为61.75 mg/L,比对照组提高82.01%。当发酵至24 h时,金属离子的添加对GSH的产量无明显促进作用。
通過搖瓶髮酵培養,研究瞭Cu2+,Mg2+,K+,Fe2+,Mn2+5種金屬離子在不同髮酵時間添加對釀酒酵母CS10515-1生產穀胱甘肽的影響。實驗結果錶明:在髮酵初期(0 h),噹K+、Mg2+、Fe2+添加量分彆為0.15、0.12、0.09 g/L時,GSH的產量分彆為88.53、52.73、41.42 mg/L,相比空白對照分彆提高瞭63.50%、36.58%和44.25%。在15 h時,噹Mg2+添加量為0.09 g/L時,GSH產量為61.75 mg/L,比對照組提高82.01%。噹髮酵至24 h時,金屬離子的添加對GSH的產量無明顯促進作用。
통과요병발효배양,연구료Cu2+,Mg2+,K+,Fe2+,Mn2+5충금속리자재불동발효시간첨가대양주효모CS10515-1생산곡광감태적영향。실험결과표명:재발효초기(0 h),당K+、Mg2+、Fe2+첨가량분별위0.15、0.12、0.09 g/L시,GSH적산량분별위88.53、52.73、41.42 mg/L,상비공백대조분별제고료63.50%、36.58%화44.25%。재15 h시,당Mg2+첨가량위0.09 g/L시,GSH산량위61.75 mg/L,비대조조제고82.01%。당발효지24 h시,금속리자적첨가대GSH적산량무명현촉진작용。
The effect of Cu2+,Mg2+,K+,Fe2+,Mn2+under different fermentation times on GSH fermentation by saccharomyces cerevisiae CS10515-1 were investigated in this paper. It was found that GSH production was 88.53 mg/L, 52.73 mg/L, 41.42 mg/L respectively, which increasing by 63.50%, 36.58%and 44.25%than the controls under the conditions of adding amount was 0.15 g/L K+,0.12 g/L Mg2+and 0.09 g/L Fe2+at 0 hour. GSH production was 61.75 mg/L, which increasing 82.01%under the conditions of 0.09 g/L Mg2+at 15h. Metal ions's adding had no effect on GSH production at 24 h.