CAJ | 학술논문

目的：了解4℃条件下储存21 d后血浆中巨细胞病毒（cytomegalovirus，CMV）DNA的稳定性。方法：收集CMV载量300～100000拷贝/mL 的30份造血干细胞移植后患者血浆，并分为低载量组、中载量组、高载量组。标本采集后立即分离血浆并进行基线（0 d）载量检测，其余血浆于4℃存储，分别在第1、2、3、7、14和21天进行实时荧光定量PCR测定。结果：将30份样本进行不同时间点的配对t检验，CMV载量均差异无显著性：第1天与第0天比较，t＝1．654，P＝0．109；第2天与第0天比较，t＝1．487，P＝0．148；第3天与第0天比较，t ＝1．609，P ＝0．118；第7天与第0天比较，t＝0．831，P＝0．413；第14天与第0天比较， t＝1．721，P＝0．096；第21天与第0天比较，t＝0．244，P＝0．244。分组比较中，第21天与第0天比较CMV载量差异无显著性（低载量组P＝0．485；中载量组P＝0．858；高载量组P＝0．949）。结论：4℃储存21 d中，血浆标本CMV载量未出现连续下降或上升趋势，且不同时间点与第0天的基线载量比较差异无显著性。
목적：료해4℃조건하저존21 d후혈장중거세포병독（cytomegalovirus，CMV）DNA적은정성。방법：수집CMV재량300～100000고패/mL 적30빈조혈간세포이식후환자혈장，병분위저재량조、중재량조、고재량조。표본채집후립즉분리혈장병진행기선（0 d）재량검측，기여혈장우4℃존저，분별재제1、2、3、7、14화21천진행실시형광정량PCR측정。결과：장30빈양본진행불동시간점적배대t검험，CMV재량균차이무현저성：제1천여제0천비교，t＝1．654，P＝0．109；제2천여제0천비교，t＝1．487，P＝0．148；제3천여제0천비교，t ＝1．609，P ＝0．118；제7천여제0천비교，t＝0．831，P＝0．413；제14천여제0천비교， t＝1．721，P＝0．096；제21천여제0천비교，t＝0．244，P＝0．244。분조비교중，제21천여제0천비교CMV재량차이무현저성（저재량조P＝0．485；중재량조P＝0．858；고재량조P＝0．949）。결론：4℃저존21 d중，혈장표본CMV재량미출현련속하강혹상승추세，차불동시간점여제0천적기선재량비교차이무현저성。
Objective To analyze CMV DNA stability of 30 EDTA plasma samples in the order of magnitude between 300 and 100 000 copies/mL over a 21 day period. Methods Thirty plasma samples were grouped into three categories according to the CMV DNA loads , including low CMV DNA contents , intermediate CMV DNA loads and high CMV DNA loads. Ten milliliters of whole blood was freshly collected from each patient. Plasma samples without hemolysis were divided into 1-ml aliquots. One aliquot was processed immediately (Day 0) for baseline PCR assays. The remaining aliquots were then processed after one , two, three, seven, 14 or 21 day of storage at 4℃. Results There was no significant difference between the mean of the difference time point in viral loads following storage at 4 ℃ by paired-samples t test, including Day 1 compared to Day 0 (t = 1.654, P =0.109), Day 2 compared to Day 0 (t = 1.487, P = 0.148), Day 3 compared to Day 0 (t = 1.609, P = 0.118), Day 7 compared to Day 0 (t=0.831, P=0.413), Day 14 compared to Day 0 (t=1.721, P=0.096), and Day 21 compared to Day 0 (t=0.244, P=0.810). Conclusion The concentration of CMV DNA in all samples stored at 4 ℃ for 21 days did not differ significantly from the baseline viral load ,and it was not observed the trend in continued degradation in different time point (Day 1, 2, 3, 7 and 14).