CAJ | 학술논문

目的：探讨过表达的野生型PTEN对体外培养的活化肝星状细胞（HSC）Akt的影响。方法：体外培养活化 HSC，以腺病毒为载体将野生型PTEN基因瞬时转染HSC；Western blot 及实时荧光定量PCR 检测HSC 的PTEN、Akt表达；Western blot检测HSC 的磷酸化Akt[p-Akt （Thr308）]表达。结果：携带野生型PTEN基因的腺病毒成功转染HSC，使活化HSC 的p-Akt （Thr308）表达下调（P＜0．01），而对HSC 的总Akt蛋白及 mRNA 表达没有影响（P＞0．50）。结论：过表达的野生型 PTEN 通过抑制 Akt 的磷酸化，负性调节活化HSC的PI3K/Akt信号转导。
목적：탐토과표체적야생형PTEN대체외배양적활화간성상세포（HSC）Akt적영향。방법：체외배양활화 HSC，이선병독위재체장야생형PTEN기인순시전염HSC；Western blot 급실시형광정량PCR 검측HSC 적PTEN、Akt표체；Western blot검측HSC 적린산화Akt[p-Akt （Thr308）]표체。결과：휴대야생형PTEN기인적선병독성공전염HSC，사활화HSC 적p-Akt （Thr308）표체하조（P＜0．01），이대HSC 적총Akt단백급 mRNA 표체몰유영향（P＞0．50）。결론：과표체적야생형 PTEN 통과억제 Akt 적린산화，부성조절활화HSC적PI3K/Akt신호전도。
Objective Using an adenoviral vector , the wild-type PTEN gene was transduced into activated hepatic stellate cell (HSC) in vitro and the phosphorylation status of Akt were investigated. Methods The wild type PTEN gene was transduced into activated HSC in vitro mediated by adenoviral vector. The expressions of PTEN and total Akt in HSC were measured by Western blot and Real-time fluorescent quantitation PCR. And the expressions of phosphorylated Akt (Thr308) in HSC was determined by Western blot. Results The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC in vitro. The over-expression of wild type PTEN resulted in the significant down-regulated expression of phosphorylated Akt (Thr308) in activated HSC (P < 0.01). But no significant defferences were found in the expression of total Akt in activated HSC at both transcriptional and translational levels(P>0.50). Conclusions The overexpression of wild-type PTEN can negatively regulate PI3K/Akt signaling transduction by inhibiting the phosphorylation of Akt in activated HSC in vitro.