CAJ | 학술논문

目的：分析小儿肺炎支原体（M P ）感染的血清检测方法。方法选取在黄冈市进行血液M P检测的呼吸道感染患儿400例，对其采取咽拭子肺炎支原体DNA聚合酶链反应（MP-DNA-PCR）和血清肺炎支原体-免疫球蛋白M抗体（抗MP-IgM），并根据不同季节、年龄段的检出率进行分析比较。结果 MP-DNA-PCR法检测的阳性率明显高于抗MP-IgM法，差异有统计学意义（P＜0．05）；8～12岁儿童检测的阳性率比其他年龄段儿童更高，各个年龄段患儿MP-DNA-PCR法阳性率均高于抗MP-IgM法，差异有统计学意义（P＜0．05）；第一季度以及第四季度阳性率明显高于第二、三季度，各个季度MP-DNA-PCR法阳性率均高于抗MP-IgM 法，差异有统计学意义（P＜0．05）。结论 M P-DNA-PCR法优于血清抗M P-IgM法，可作为标准检测方法为临床诊断服务。
목적：분석소인폐염지원체（M P ）감염적혈청검측방법。방법선취재황강시진행혈액M P검측적호흡도감염환인400례，대기채취인식자폐염지원체DNA취합매련반응（MP-DNA-PCR）화혈청폐염지원체-면역구단백M항체（항MP-IgM），병근거불동계절、년령단적검출솔진행분석비교。결과 MP-DNA-PCR법검측적양성솔명현고우항MP-IgM법，차이유통계학의의（P＜0．05）；8～12세인동검측적양성솔비기타년령단인동경고，각개년령단환인MP-DNA-PCR법양성솔균고우항MP-IgM법，차이유통계학의의（P＜0．05）；제일계도이급제사계도양성솔명현고우제이、삼계도，각개계도MP-DNA-PCR법양성솔균고우항MP-IgM 법，차이유통계학의의（P＜0．05）。결론 M P-DNA-PCR법우우혈청항M P-IgM법，가작위표준검측방법위림상진단복무。
Objective To analyze the serum markers in children with infection of Mycoplasma pneumoniae (MP) .Methods Throat swabs and serum samples were collected from 400 children with infection of MP ,and detec-ted for MP DNA by polymerase chain reaction(PCR) and anti-MP-IgM antibody .All results were analyzed according to seasons and ages .Results The positive rate of MP-DNA-PCR detection was significantly higher than anti-MP-IgM detection(P<0 .05) .Of the both two methods ,the positive rate in children at age of 8-12 year-old was higher than those in children at other ages ,and of children at different ages ,the positive rates of MP-DNA-PCR detection were significantly higher than anti-MP-IgM detection(P< 0 .05) .The positive rates of the first and fourth quarter were significantly higher than those of the second and third quarter ,and of each quarter ,the positive rates of MP-DNA-PCR detection were significantly higher than anti-MP-IgM detection(P< 0 .05) .Conclusion MP-DNA-PCR detection might be superior to serum anti-M P-IgM detection ,and the former could be used as a standard test for clini-cal diagnosis .