CAJ | 학술논문

目的：通过对人肝癌细胞（HepG2）细胞膜上乙型肝炎病毒（HBV）前S1区（preS1）的结合蛋白进行分离和质谱分析，寻找与HBV黏附相关的特异受体。方法以免疫磁珠法分离与preS1肽段结合的HepG2膜蛋白，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离结合蛋白，选取目的条带进行LC-MS/MS质谱分析及数据库检索。结果 HepG2细胞与preS1结合的蛋白，通过SDS-PAGE电泳分离出了16条带，选取HepG2与preS1结合蛋白分离出重复性较好的6条带，质谱分析出14种蛋白。结论质谱分析的蛋白主要是与物质运输、细胞信号转导、抗原提呈、免疫调节以及能量代谢相关的蛋白。
목적：통과대인간암세포（HepG2）세포막상을형간염병독（HBV）전S1구（preS1）적결합단백진행분리화질보분석，심조여HBV점부상관적특이수체。방법이면역자주법분리여preS1태단결합적HepG2막단백，통과십이완기류산납-취병희선알응효전영（SDS-PAGE）분리결합단백，선취목적조대진행LC-MS/MS질보분석급수거고검색。결과 HepG2세포여preS1결합적단백，통과SDS-PAGE전영분리출료16조대，선취HepG2여preS1결합단백분리출중복성교호적6조대，질보분석출14충단백。결론질보분석적단백주요시여물질운수、세포신호전도、항원제정、면역조절이급능량대사상관적단백。
Objective To seek the specific receptors associated with hepatitis B virus (HBV) adhesion by separating the binding protein of the HBV preS1 region in HepG2 and performing the mass spectrometry .Methods The immunomagnetic bead method was adopted to separate HepG2 membrane protein combined with preS1 peptide fragment and the binding protein was separated by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ,then the destination strips was analyzed by LC-MS/MS mass spectrometry and retrieved by the database .Results 16 bands were separated from HepG2 membrane proteins combined with preS1 by SDS-PAGE ;14 kinds of proteins were identified from 6 bands with better repeatability separated from HepG 2 membrane proteins combined with preS1 .Conclusion Protein analyzed by the mass spectrometry is mainly related with the material transport , cellular signal transduction ,antigen presentation ,immune regulation and energy metabolism .