CAJ | 학술논문

目的：观察隐丹参酮（CTS）与多奈哌齐（DON）合用对β-淀粉样蛋白（Aβ）损伤SH-SY5Y细胞的保护作用，并探讨其可能的机制。方法体外培养SH-SY5Y细胞，建立阿尔茨海默病（AD）细胞模型，采用MTT 法检测细胞存活率，Hoechst 荧光染色检测细胞凋亡，Western blot检测Bcl-2和Bax的表达。结果 CTS、DON及联合用药均能明显减轻Aβ对SH-SY5Y细胞的损伤，提高细胞的存活率，显著上调Bc1-2蛋白表达，减少Bax 蛋白表达，抑制细胞凋亡。CTS与DON合用其抑制细胞凋亡的效果显著强于CTS与DON单用，差异有统计学意义（P＜0．05）。结论 CTS与DON合用对Aβ损伤SH-SY5Y细胞有协同保护作用，其机制可能与协同调节凋亡相关基因Bcl-2蛋白家族的表达有关。
목적：관찰은단삼동（CTS）여다내고제（DON）합용대β-정분양단백（Aβ）손상SH-SY5Y세포적보호작용，병탐토기가능적궤제。방법체외배양SH-SY5Y세포，건립아이자해묵병（AD）세포모형，채용MTT 법검측세포존활솔，Hoechst 형광염색검측세포조망，Western blot검측Bcl-2화Bax적표체。결과 CTS、DON급연합용약균능명현감경Aβ대SH-SY5Y세포적손상，제고세포적존활솔，현저상조Bc1-2단백표체，감소Bax 단백표체，억제세포조망。CTS여DON합용기억제세포조망적효과현저강우CTS여DON단용，차이유통계학의의（P＜0．05）。결론 CTS여DON합용대Aβ손상SH-SY5Y세포유협동보호작용，기궤제가능여협동조절조망상관기인Bcl-2단백가족적표체유관。
Objective To investigate the protective effect of cryptotanshinone (CTS) and donepezil(DON) on amyloid-βprotein (Aβ)-induced apoptosis in SH-SY5Y cells .Methods SH-SY5Y cells were cultured in vitro for establishing the Alzheimer disease (AD) model .The cell viability was detected by the MTT assay .The apoptosis rate was measured by Hoechst 33342 and the expres-sion of Bcl-2 and Bax was detected by Western blot .Results CTS ,DON and their combination could obviously alleviate Aβ-caused injury of SH-SY5Y cells ,increase the cell survival rate ,remarkably up-regulate the expression of Bcl-2 protein ,decrease the expres-sion of Bax protein and inhibit the apoptosis .The effect of the CTS and DON combination for inhibiting apoptosis was significantly stronger than that of the single use of CTS and DON ,the difference had statistical significance(P<0 .05) .Conclusion The combi-nation of CTS and DON has the synergistic protective effect on Aβ-caused injury in SH-SY5Y cells ,its mechanisms may be related with the cooperation regulation of the expression of apoptosis related gene Bcl-2 protein family .