CAJ | 학술논문

目的：初步筛选出肝母细胞瘤（HB）中的肿瘤干细胞（NSC）相关表面标记组合，并了解NSC的分布。方法选择入住江西省儿童医院并接受手术的 HB患儿，采用免疫组织化学染色的方法，观察干细胞相关标记物CD34、T hy-1、c-kit、CD56和干细胞生长因子（SCF），在 HB组织及距肿瘤组织边缘3 cm以外的正常肝脏组织的表达和分布。结果 Thy-1、c-kit散在分布于HB组织中，主要集中在汇管区，而正常肝脏组织中不表达；CD34及SCF在HB中的表达明显高于正常肝脏组织（P＜0．05），其中CD34主要在血管内皮系统中分布，SCF主要表达在汇管区；CD56表达于成簇的神经纤维组织中，在 HB及正常肝脏组织中表达差异无统计学意义（P＞0．05）。结论不同干细胞相关表面标志物分布于 HB组织中，并且集中表达在特定区域。表达 Thy-1/c-kit阳性细胞可能对 HB的发生起一定作用。
목적：초보사선출간모세포류（HB）중적종류간세포（NSC）상관표면표기조합，병료해NSC적분포。방법선택입주강서성인동의원병접수수술적 HB환인，채용면역조직화학염색적방법，관찰간세포상관표기물CD34、T hy-1、c-kit、CD56화간세포생장인자（SCF），재 HB조직급거종류조직변연3 cm이외적정상간장조직적표체화분포。결과 Thy-1、c-kit산재분포우HB조직중，주요집중재회관구，이정상간장조직중불표체；CD34급SCF재HB중적표체명현고우정상간장조직（P＜0．05），기중CD34주요재혈관내피계통중분포，SCF주요표체재회관구；CD56표체우성족적신경섬유조직중，재 HB급정상간장조직중표체차이무통계학의의（P＞0．05）。결론불동간세포상관표면표지물분포우 HB조직중，병차집중표체재특정구역。표체 Thy-1/c-kit양성세포가능대 HB적발생기일정작용。
Objective To preliminarily screen the neoplastic stem cell (NSC) related surface markers combination and to under-stand the NSC distribution in hepatoblastoma(HB) .Methods The children cases of HB undergone the surgical therapy in the Jian-gxi Provincial Children′s Hospital were selected .The immunohistochemical method was adopted for observing the expression and distribution of NSC-related markers CD34 ,Thy-1 ,c-kit ,CD56 and stem cell factor(SCF) in the HB tissue and the normal hepatic tissue away from the edge of tumor tissue 3cm outside .Results Thy-1 and c-kit were sporadically distributed in the HB tissue and mainly focued on the portal area ,but did not exressed in the normal liver tissue ;the expression of CD34 and SCF in HB was signifi-cantly higher than that in the normal liver tissue(P<0 .05) ,in which CD34 was mainly distributed in the endothelial system and SCF was mainly expressed in the portal area ;CD56 was expressed in the tissue of the clustered nerve fibers ,the expression differ-ence between them had no statistical significance (P>0 .05) .Conclusion The different NSC related surface markers are distributed in the HB tissue and focus on the specific areas .The positive cells of Thy-1/c-kit expression may play a role in the HB occurrence .