CAJ | 학술논문

目的：观察携带有人β神经生长因子（β-NGF）重组质粒的骨髓基质干细胞（BMSC）的生物学特性。方法培养小鼠BMSC ，采用脂质体LipofectamineTM 2000（Lip2000）转染方法将含有人β-NGF基因的重组质粒转染至含有绿色荧光蛋白（GFP）基因的小鼠BMSC中，利用酶联免疫吸附测定（ELISA）的方法检测其β-NGF蛋白的表达水平。结果采用脂质体Lip2000转染方法能成功地将β-NGF重组质粒转染至BMSC ，转染后细胞出现β-NGF基因的表达，并且其表达的β-NGF蛋白能有效地保护转染过程中对细胞的损害。结论经转染后携带β-NGF基因质粒的小鼠BMSC仍有增殖、分化能力，并且具有了β-NGF蛋白的表达能力。
목적：관찰휴대유인β신경생장인자（β-NGF）중조질립적골수기질간세포（BMSC）적생물학특성。방법배양소서BMSC ，채용지질체LipofectamineTM 2000（Lip2000）전염방법장함유인β-NGF기인적중조질립전염지함유록색형광단백（GFP）기인적소서BMSC중，이용매련면역흡부측정（ELISA）적방법검측기β-NGF단백적표체수평。결과채용지질체Lip2000전염방법능성공지장β-NGF중조질립전염지BMSC ，전염후세포출현β-NGF기인적표체，병차기표체적β-NGF단백능유효지보호전염과정중대세포적손해。결론경전염후휴대β-NGF기인질립적소서BMSC잉유증식、분화능력，병차구유료β-NGF단백적표체능력。
Objective To investigate the biological features of the mouse bone marrow stromal stem cells (BMSCs) transfected by humanβnerve growth factor(β-NGF) .Methods BMSCs of GFP transgenic mouse were isolated and cultured .Theβ-NGF re-combinant plasmid vectors were transferred into the cultured BMSCs by LipofectamineTM 2000 .The expression of β-NGF was detec-ted with ELISA .Results The β-NGF recombinant plasmid vectors were successfully transferred into BMSCs of GFP transgenic mouse ,the expression of β-NGF in transfected cells appeared .In addition ,the expression ofβ-NGF could effectively protect the BM-SCs which were injured in transfection process .Conclusion BMSCs of GFP transgenic mouse after transfecton with human β-NGF have the proliferation and differentiation capacity ,and possess the expression ability of β-NGF protein .