CAJ | 학술논문

目的：建立大鼠肺组织羟脯氨酸（HYP）水平测定方法，以评价大鼠肺组织纤维化程度。方法比较不同酸水解时间、氧化时间、显色时间等对大鼠肺组织 HYP水平测定结果的影响，据此建立大鼠肺组织 HYP水平测定方法，并初步应用于博莱霉素诱导大鼠肺纤维化 HYP水平的测定。结果通过优化得出HYP水平测定最佳条件为110℃7．50 mol盐酸（HCL）水解16 h ，常温氧化10 min ，60℃显色25 min。本方法灵敏度为0．067μg/mL ，回收率为88．85％～110．88％，平均 CV为4．70％～6．60％。该法应用于博莱霉素诱导大鼠肺纤维化研究中，模型组 HYP水平明显高于对照组。结论该法灵敏度与回收率均较高、重现性较好，可用作临床对肺纤维化程度判定的定量方法。
목적：건립대서폐조직간포안산（HYP）수평측정방법，이평개대서폐조직섬유화정도。방법비교불동산수해시간、양화시간、현색시간등대대서폐조직 HYP수평측정결과적영향，거차건립대서폐조직 HYP수평측정방법，병초보응용우박래매소유도대서폐섬유화 HYP수평적측정。결과통과우화득출HYP수평측정최가조건위110℃7．50 mol염산（HCL）수해16 h ，상온양화10 min ，60℃현색25 min。본방법령민도위0．067μg/mL ，회수솔위88．85％～110．88％，평균 CV위4．70％～6．60％。해법응용우박래매소유도대서폐섬유화연구중，모형조 HYP수평명현고우대조조。결론해법령민도여회수솔균교고、중현성교호，가용작림상대폐섬유화정도판정적정량방법。
Objective To establish the assay method of hydroxyproline(HYP) level in rat lung tissue for evaluating the lung fi-brosis degree .Methods The impact of different acid hydrolyzable time ,oxidative time ,developing time on the assay results of the HYP level in rat lung tissue was studied .On this basis ,the assay method for determining the HYP level was established and prelim-inarily applied in the detection of the HYP level in rat lung tissue .Results The optimal condition for the HYP level detection in rat lung tissue was as follows :7 .50 mol HCL was hydrolyzed for 16 h at 110 ℃ ,oxidized for 10 min at room temperature and devel-oped for 25min at 60℃ .The sensitivity of this method was 0 .067μg/mL .The recovery rate and the average CV of this method were 88 .85% -110 .88% and 4 .70% -6 .60% ,respectively .In the study of bleomycin induced rat lung fibrosis ,the HYP level of the model group was obviously higher than that of the control group .Conclusion This method has high sensitivity ,high recovery rate and good reproducibility ,and may be used as a reliable quantitative method to judge the lung fibrosis level in clinic .