CAJ | 학술논문

目的：探讨花生根乙醇提取物对丙酸睾酮（T P ）诱导未去势成年大鼠前列腺增生的抑制作用及其机制。方法将60只SD大鼠随机分为对照组、模型组及花生根乙醇提取物高、中、低剂量治疗组，其中对照组大鼠正常喂饲，不用药；模型组及各治疗组皮下注射TP5mL·kg -1·d-1，高、中、低剂量治疗组同时灌胃不同剂量的花生根乙醇提取物（剂量分别为10mL·kg -1· d-1、5mL·kg -1·d-1、1mL·kg -1·d-1），连续14d。各组均于第15d处死，取前列腺、精囊和睾丸组织并称重，计算各腺体指数[腺体湿质量（mg）/体质量（g）]；采用免疫组织化学法检测各组大鼠前列腺组织Bcl-2和Bax蛋白的表达。结果花生根乙醇提取物高、中剂量治疗组与模型组比较，前列腺指数差异均有统计学意义（P＜0．01）；低剂量组与模型组比较，差异无统计学意义（P＞0．05），花生根乙醇提取物抑制前列腺增生作用具有量效关系。花生根乙醇提取物高、中、低剂量组与模型组比较，睾丸指数、精囊指数、体质量变化差异无统计学意义（ P＞0．05）。花生根乙醇提取物高、中、低剂量治疗组Bcl-2和Bax 蛋白表达率与模型组比较，差异有统计学意义（ P＜0．05）；与对照组Bcl-2和Bax 蛋白表达比较，差异无统计学意义（ P＞0．05）。结论花生根乙醇提取物具有良好的抑制前列腺增生作用，其机制可能是通过调节凋亡基因的Bcl-2和Bax蛋白比例平衡关系，促进良性前列腺增生（BPH）的细胞凋亡达到治疗的效果。
목적：탐토화생근을순제취물대병산고동（T P ）유도미거세성년대서전렬선증생적억제작용급기궤제。방법장60지SD대서수궤분위대조조、모형조급화생근을순제취물고、중、저제량치료조，기중대조조대서정상위사，불용약；모형조급각치료조피하주사TP5mL·kg -1·d-1，고、중、저제량치료조동시관위불동제량적화생근을순제취물（제량분별위10mL·kg -1· d-1、5mL·kg -1·d-1、1mL·kg -1·d-1），련속14d。각조균우제15d처사，취전렬선、정낭화고환조직병칭중，계산각선체지수[선체습질량（mg）/체질량（g）]；채용면역조직화학법검측각조대서전렬선조직Bcl-2화Bax단백적표체。결과화생근을순제취물고、중제량치료조여모형조비교，전렬선지수차이균유통계학의의（P＜0．01）；저제량조여모형조비교，차이무통계학의의（P＞0．05），화생근을순제취물억제전렬선증생작용구유량효관계。화생근을순제취물고、중、저제량조여모형조비교，고환지수、정낭지수、체질량변화차이무통계학의의（ P＞0．05）。화생근을순제취물고、중、저제량치료조Bcl-2화Bax 단백표체솔여모형조비교，차이유통계학의의（ P＜0．05）；여대조조Bcl-2화Bax 단백표체비교，차이무통계학의의（ P＞0．05）。결론화생근을순제취물구유량호적억제전렬선증생작용，기궤제가능시통과조절조망기인적Bcl-2화Bax단백비례평형관계，촉진량성전렬선증생（BPH）적세포조망체도치료적효과。
Objective To explore the inhibitory effect of the ethanol extract from peanut root on the non-castration adult rat prostate hyperplasia induced by testosterone propionate and its mechanism .Methods 60 SD rats were randomly divided into the control ,model and high ,middle and low dose of peanut root ethanol extract treatment groups ,among them ,the control group was normally fed without medication ;the model group was subcutaneously injected by testosterone propionate (TP ,5 mL/kg/d) and simultaneously gavaged subcutaneous injection with peanut root ethanol extract (10 mL/kg/d ,5 mLg/kg/d or 1 mL/kg/d) for suc-cessive 14 d .The rats in various groups were killed on 15 d and their prostate ,spermatophore and testicle tissues were separated and weighed .The ratio of gland/body-weight(mg/g) was calculated .The expressions of Bcl-2 and Bax proteins in the prostate tissues were detected by immunohistochemistry .Results There was statistical difference in the indexes of prostate between the treatment groups(high and middle dose) and the model group(P<0 .01) ,while there was no statistical difference between the low dose group and the model group(P>0 .05) .This result showed that there was a dose-effect relationship between the inhibition effect on rat prostate hyperplasia and the ethanol extracts from peanut root .There was no statistical differences between the model group and the treatment groups in the indexes of testicle ,spermatophore or the change of weight (P>0 .05) .There was statistical difference be-tween the control group or model group and the treatment group in the expression of Bcl-2 or Bax protein(P<0 .05) ,while no sta-tistical difference between the treatment group and the control group (P>0 .05) .Conclusion Ethanol extract from peanut root has good inhibiting effect on prostate hyperplasia and its mechanism could reach the curative effect by regulating the balance relation be-tween apoptosis gene Bcl-2 and Bax protein proportion and promoting apopitosis of benign prostatic hyperplasia .