CAJ | 학술논문

目的：探讨mecA基因在临床分离金黄色葡萄球菌（SA ）中的表达水平与耐药的关系。方法收集临床分离的SA菌株186株，采用纸片扩散法（K-B法）进行药敏试验，并提取DNA ，运用PCR扩增mecA基因。结果耐甲氧西林金黄色葡萄球菌（MRSA）的检出率为30．65％（57/186），其中56株 MRSA和129株甲氧西林敏感金黄色葡萄球菌（MSSA）中有10株检出mecA基因阳性；除对万古霉素、利奈唑胺敏感外，MRSA对其他抗菌药物耐药率均高于MSSA ，携带mecA基因的菌株抗菌药物耐药率高于不携带mecA基因的菌株，两组差异有统计学意义（P＜0．05）。结论 SA临床分离菌株中含mecA基因的菌株对多种抗菌药物耐药，可见mecA基因在SA的耐药机制中发挥着重要作用。
목적：탐토mecA기인재림상분리금황색포도구균（SA ）중적표체수평여내약적관계。방법수집림상분리적SA균주186주，채용지편확산법（K-B법）진행약민시험，병제취DNA ，운용PCR확증mecA기인。결과내갑양서림금황색포도구균（MRSA）적검출솔위30．65％（57/186），기중56주 MRSA화129주갑양서림민감금황색포도구균（MSSA）중유10주검출mecA기인양성；제대만고매소、리내서알민감외，MRSA대기타항균약물내약솔균고우MSSA ，휴대mecA기인적균주항균약물내약솔고우불휴대mecA기인적균주，량조차이유통계학의의（P＜0．05）。결론 SA림상분리균주중함mecA기인적균주대다충항균약물내약，가견mecA기인재SA적내약궤제중발휘착중요작용。
Objective To investigate the mecA gene expression lvevl in clinically isolated Staphylococcus aureus (SA) strains and its correlation with drug resistance .Methods Clinically isolated 186 SA strains were collected .The K-P method was adopted to conduct the durg sensitivity test .Then DNA of these strains was extracted and the mecA gene was amplified by using PCR .Results The detection rate of methicillin-resistant SA(MRSA) was 30 .65% (57/186) ,the positive mecA gene was detected in 56 strains of MRSA and 10 strains of methicillin susceptible S .aureus(MSSA) among 129 strains of MSSA ;except susceptible to vancomycin and linezolid ,the resistance rate of MRSA to ther antibacterial durgs were higher than that of MSSA ,the resistance rate to antibac-terial durgs in the strains carrying mecA gene was higher than that in the strains without carrying mecA gene ,ther difference be-tween them had statistical significance (P<0 .05) .Conclusion Clinically isolated SA strains carrying mecA gene are resistant to multiple antibacterial drugs ,which indicating that mecA gene play an important role in SA drug-resistance mechanism .