CAJ | 학술논문

目的：探讨色素上皮衍生因子（PEDF）对小鼠骨髓源性树突细胞（BMDCs）表型及其免疫功能的影响。方法从小鼠骨髓中分离单个核细胞（MNC），用小鼠重组粒细胞巨噬细胞集落刺激因子（rmGM-CSF）、小鼠重组白细胞介素-4（rmIL-4）培养5 d后将其分为5组，实验组分别加入50、100、200 ng/mL PEDF ，阳性对照中加1μg/mL脂多糖（LPS），阴性对照中加等量的RP-M I1640培养基（对照组），继续培养3 d。流式细胞术（FCM ）分析DCs表面CD11c、CD80、CD86表达情况，混合淋巴细胞反应（MLR）检测BMDCs对T细胞的刺激能力，ELISA法检测其培养上清液中IL-12水平。结果 PEDF处理后的BMDCs明显上调了CD11c、CD80和CD86的表达，并能明显增强 T 细胞的活性，促进其分泌 IL-12。结论 PEDF可明显上调体外培养的小鼠BMDCs免疫标记分子的表达，并增强其免疫活性。
목적：탐토색소상피연생인자（PEDF）대소서골수원성수돌세포（BMDCs）표형급기면역공능적영향。방법종소서골수중분리단개핵세포（MNC），용소서중조립세포거서세포집락자격인자（rmGM-CSF）、소서중조백세포개소-4（rmIL-4）배양5 d후장기분위5조，실험조분별가입50、100、200 ng/mL PEDF ，양성대조중가1μg/mL지다당（LPS），음성대조중가등량적RP-M I1640배양기（대조조），계속배양3 d。류식세포술（FCM ）분석DCs표면CD11c、CD80、CD86표체정황，혼합림파세포반응（MLR）검측BMDCs대T세포적자격능력，ELISA법검측기배양상청액중IL-12수평。결과 PEDF처리후적BMDCs명현상조료CD11c、CD80화CD86적표체，병능명현증강 T 세포적활성，촉진기분비 IL-12。결론 PEDF가명현상조체외배양적소서BMDCs면역표기분자적표체，병증강기면역활성。
Objective To explore the effects of pigmentary epithelium derived factor (PDEF) on the phenotypic and immunologic function of murine-derived dentritic cells(BMDCs) .Methods Mononuclear cells(MNCs) isolated from murine bone marrow were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 for 5 d ,and were divided into five groups .MNCs were stimulated for 3 d with either 50 ,100 ,200ng/mL PEDF ,1 μg/mL LPS(positive control) or RPMI1640(negative control) .The expression of CD11c ,CD80 and CD86 on DCs surface were analyzed by the fluorescence activated cell sorting (FCM ) .The ability of PEDF-induced BMDCs to stimulated T cell maturation were determined by the CCK-8 method and the level of IL-12 in the culture supernatant was detected by ELISA .Results The PEDF-treated BMDCs expressed high levels of CD11c ,CD80 and CD86 ,enhanced the immunolog-ical activities of T lymphocyte and its secretion of IL-12 when compared with untreated DCs .Conclusion PEDF can significantly up-regulate the expression of DCs immunological labelled molecule in in vitro cultured murine and increase its immunological com-petence .