CAJ | 학술논문

目的：探讨同型半胱氨酸（Hcy）对肝细胞增殖及细胞周期素D1（CyclinD1）、丙氨酸氨基转移酶（ALT）和天门冬氨酸氨基转移酶（AST ）表达的影响。方法将 HL-7702细胞体外常规培养，分别用0、50、100、200、500μmol/L Hcy刺激细胞，分别于刺激后6、12、24 h采用M T T法检测肝细胞的增殖情况；采用荧光定量 PCR（RT-qPCR）检测CyclinD1 mRNA的表达；采用微板法检测各组细胞培养液中ALT和 AST 的变化。结果不同浓度 Hcy刺激肝细胞不同时间后，细胞的增殖受到抑制，其中100、200、500μmol/L Hcy组与对照组比较差异有统计学意义（P＜0．01），24 h作用最明显；不同浓度 Hcy刺激肝细胞24 h后， CyclinD1 mRNA的表达显著下降（P＜0．01），细胞培养液中ALT和AST 水平显著升高（P＜0．01）。结论 Hcy可以抑制肝细胞增殖，并引起CyclinD1的mRNA表达下降，ALT和AST释放增多。
목적：탐토동형반광안산（Hcy）대간세포증식급세포주기소D1（CyclinD1）、병안산안기전이매（ALT）화천문동안산안기전이매（AST ）표체적영향。방법장 HL-7702세포체외상규배양，분별용0、50、100、200、500μmol/L Hcy자격세포，분별우자격후6、12、24 h채용M T T법검측간세포적증식정황；채용형광정량 PCR（RT-qPCR）검측CyclinD1 mRNA적표체；채용미판법검측각조세포배양액중ALT화 AST 적변화。결과불동농도 Hcy자격간세포불동시간후，세포적증식수도억제，기중100、200、500μmol/L Hcy조여대조조비교차이유통계학의의（P＜0．01），24 h작용최명현；불동농도 Hcy자격간세포24 h후， CyclinD1 mRNA적표체현저하강（P＜0．01），세포배양액중ALT화AST 수평현저승고（P＜0．01）。결론 Hcy가이억제간세포증식，병인기CyclinD1적mRNA표체하강，ALT화AST석방증다。
Objective To explore the effects of homoysteine(Hcy) on the proliferation of hepatic cells and the expression of Cy-clinD1 ,ALT and AST .Methods HL-7702 hepatic cells were routinely cultured in vitro and irritated with Hcy at a concentration of 0 ,50 ,100 ,200 ,500μmol/L ,respectively .The proliferation of hepatic cells was measured by MTT at 6 ,12 ,24h after irritation .The expression of CyclinD1 mRNA was detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR) .The chan-ges of ALT and AST in the cells culture medium of each group were determined by the microplate method .Results The prolifera-tion of hepatic cells was inhibited by different concentrations of Hcy ,in which the proliferation rates irritated by Hcy at the concen-trations of 100 ,200 ,500μmol/L were lower than that in control group with statistical difference (P<0 .01) ,and the effect was most markedly at 24h after irritation(P<0 .01);compared with the control group ,when hepatic cells were irritated by Hcy at the concen-trations of 100 ,200 ,500μmol/L for 24h ,the mRNA expression of CyclinD1 was decreased(P<0 .01) ,meanwhile the levels of ALT and AST were significantly increased(P<0 .01) .Conclusion Hcy can inhibit the proliferation of hepatic cells ,cause the decrease of CyclinD1 mRNA expression and result in the increase of ALT and AST release .