CAJ | 학술논문

目的：探讨前列腺素E2（PGE2）与成骨细胞力学敏感性的关系，并对其细胞水平的作用机制进行分析。方法使用16，16-二甲基PGE2（dmPGE2）对成骨细胞株（MC3T3-E1）进行预处理，随后观察其对细胞内钙离子信号的调控作用；并采用蛋白激酶A（PKA）信号通路的激活剂与阻滞剂检验PKA信号通路在此调控过程中的参与作用。结果相对于空白处理组，dmPGE2预处理显著提升了细胞中由水肿胀刺激引起的钙离子信号强度。该作用被 PK A信号通路的激活剂8-溴腺苷-3，5-环单磷酸钠（8br-cAMP）所模拟；且被PKA多肽阻滞剂（PKI）阻断。结论 dmPGE2通过激活PKA信号通路上调MC3T3-E1细胞中由水肿胀刺激引起的钙离子响应，为解释PGE2在骨组织中的促合成作用提供了重要的细胞学基础。
목적：탐토전렬선소E2（PGE2）여성골세포역학민감성적관계，병대기세포수평적작용궤제진행분석。방법사용16，16-이갑기PGE2（dmPGE2）대성골세포주（MC3T3-E1）진행예처리，수후관찰기대세포내개리자신호적조공작용；병채용단백격매A（PKA）신호통로적격활제여조체제검험PKA신호통로재차조공과정중적삼여작용。결과상대우공백처리조，dmPGE2예처리현저제승료세포중유수종창자격인기적개리자신호강도。해작용피 PK A신호통로적격활제8-추선감-3，5-배단린산납（8br-cAMP）소모의；차피PKA다태조체제（PKI）조단。결론 dmPGE2통과격활PKA신호통로상조MC3T3-E1세포중유수종창자격인기적개리자향응，위해석PGE2재골조직중적촉합성작용제공료중요적세포학기출。
Objective To explore the relation between prostaglandin E2(PGE2) and the mechanosensitivity of osteoblasts and to analyze its action mechanism in cellular level .Methods Osteoblasts cell line MC3T3-E1 cells were performed the pre-treatment by using 16 ,16-dimethyl prostaglandin E2(dmPGE2) .Then the regulating effect of PGE2 on intracellular calcium ion signal was ob-served;the activator agent and inhibitor of the protein kinase A (PKA) signal path were adopted to detect the participating effect of the PKA signal path in the regulating process .Results Compared with the blank treatment group ,dmPGE2 pre-treatment signifi-cantly increased the calcium ion signal intensity induced by hypotonic swelling stimulation in MC 3T3-E1 cells .This effect of dmPGE2 was mimicked by 8-bromo-cAMP(8br-cAMP) ,an activator of PKA pathway ;but blocked by PKI ,an inhibitor of PKA pathway .Conclusion dmPGE2 is able to up-regulate the calcium response induced by hypotonic swelling stimulation by activating PKA pathway ,which provides an important cellular mechanism for explaining PGE2′s anabolic effect in bone tissue .