CAJ | 학술논문

目的：探讨肺癌组织中微RNA（miRNA）的表达谱以及在CD133＋ A549中的表达。方法取肺腺癌患者的肺癌组织标本和癌旁组织标本，进行 miRNA 表达芯片分析。利用流式细胞术（FCM ）分选并培养 CD133＋ A549细胞2周，检测CD133＋、CD44＋表达分析并应用实时荧光定量PCR检测miRNA-892b和miRNA-4686在CD133＋ A549细胞中的表达情况。结果肺癌组织中有27个miRNA表达较癌旁组织中的表达上调（P＜0．05），6个miRNA的表达较癌旁组织中的表达下调（P＜0．05）。CD133＋ A549细胞CD133＋、CD44＋双阳性表达率为75．0％。CD133＋ A549细胞中miRNA-892b和miRNA-4686的表达显著高于A549细胞（P＜0．01）。结论肺腺癌组织中 miRNA存在异常表达谱，miRNA-892b和miRNA-4686可能在维持CD133＋肺癌细胞生物学特性中发挥着一定的作用。
목적：탐토폐암조직중미RNA（miRNA）적표체보이급재CD133＋ A549중적표체。방법취폐선암환자적폐암조직표본화암방조직표본，진행 miRNA 표체심편분석。이용류식세포술（FCM ）분선병배양 CD133＋ A549세포2주，검측CD133＋、CD44＋표체분석병응용실시형광정량PCR검측miRNA-892b화miRNA-4686재CD133＋ A549세포중적표체정황。결과폐암조직중유27개miRNA표체교암방조직중적표체상조（P＜0．05），6개miRNA적표체교암방조직중적표체하조（P＜0．05）。CD133＋ A549세포CD133＋、CD44＋쌍양성표체솔위75．0％。CD133＋ A549세포중miRNA-892b화miRNA-4686적표체현저고우A549세포（P＜0．01）。결론폐선암조직중 miRNA존재이상표체보，miRNA-892b화miRNA-4686가능재유지CD133＋폐암세포생물학특성중발휘착일정적작용。
Objective To study the expression profile of microRNAs in the lung adenocarcinoma tissue and its expression in CD133+ lung cancer cells .Methods The specimens of adenocarcinoma tissue and pericarcinomatous tissue were collected for con-ducting the microRNA expression chip analysis .The flow cytometry(FCM ) was adopted to sort and culture CD133+ A549 cells in serum-free medium for 2 weeks .The positive rate of CD133+CD44+ in those cells was assayed .The expression of miRNA-892b and miRNA-4686 in CD133+ A549 cells were detected by real-time qPCR .Results Compared with the pericarcinomatous tissue ,27 miRNAs expressions in the lung adenocarcinoma tissue were up-regulated(P<0 .05) and 6 miRNAs expressions were down-regula-ted(P<0 .05) .The positive rate of cancer stem like cells CD 133+CD44+ was 75 .0% .The expression of miRNA-892b and miRNA-4686 in CD133+ A549 cells was higher than A549 cells(P<0 .01) .Conclusion Abnormal expression profile of miRNAs in lung ad-enocarcinoma tissue ,and miRNA-892b and miRNA-4686 may play a certain role to maintain the biological characteristics of CD133+ lung cancer cells .