CAJ | 학술논문

目的：建立测定注射用门冬氨酸钾镁中无水门冬氨酸钾镁含量的方法。方法：采用离子色谱法，色谱柱为CS12A（4 mm ×250 mm），保护柱为CG12A（4 mm ×50 mm）；电导检测器，阳离子抑制器；流动相为25 mmol· L-1甲烷磺酸溶液，流量为1 mL· min-1；柱温30℃。结果：无水门冬氨酸钾在22.08～441.64μg· mL-1范围内，色谱峰面积与其浓度成良好线性关系（Y＝0.0377X-0.0145，R2＝1），回收率为100.1％，RSD为0.7％；无水门冬氨酸镁在24.09～481.84μg· mL-1范围内，色谱峰面积与其浓度成良好线性关系（Y＝0.0409X-0.0088，R2＝1），回收率为100.9％，RSD为0.4％。结论：本方法可简便、快速、准确地对注射用门冬氨酸钾镁中无水门冬氨酸钾镁含量进行测定。
목적：건립측정주사용문동안산갑미중무수문동안산갑미함량적방법。방법：채용리자색보법，색보주위CS12A（4 mm ×250 mm），보호주위CG12A（4 mm ×50 mm）；전도검측기，양리자억제기；류동상위25 mmol· L-1갑완광산용액，류량위1 mL· min-1；주온30℃。결과：무수문동안산갑재22.08～441.64μg· mL-1범위내，색보봉면적여기농도성량호선성관계（Y＝0.0377X-0.0145，R2＝1），회수솔위100.1％，RSD위0.7％；무수문동안산미재24.09～481.84μg· mL-1범위내，색보봉면적여기농도성량호선성관계（Y＝0.0409X-0.0088，R2＝1），회수솔위100.9％，RSD위0.4％。결론：본방법가간편、쾌속、준학지대주사용문동안산갑미중무수문동안산갑미함량진행측정。
Objective:To establish an assay method of potassium aspartate and magnesium aspartate in potassium aspartate and magnesi -um aspartate for injection by ion chromatography (IC).Methods:The assay was performed on CS12A column(4 mm ×250 mm)and CG12A guard column (4 mm ×50 mm) with cationic suppressor and electrolytic conductivity detector.The mobile phase was 25 mmol · L-1 methanesulfonic acid solution with 1 mL· min-1 flow rate.The temperature of column was 30 ℃.Results: Within 22.08-441.64 μg· mL-1 potassium aspartate had a good linearity (Y=0.0377X-0.0145,R2 =1),the average recovery was 100.1% with RSD of 0.7%.Within 24.09-481.84 μg· mL-1 magnesium aspartate had a good linearity (Y=0.0409X-0.0088,R2 =1),the av-erage recovery was 100.9%with RSD of 0.4%.Conclusion:The method is proved to be simple ,rapid and sensitive for evaluating the quality of potassium aspartate and magnesium aspartate in potassium aspartate and magnesium aspartate for injection .