CAJ | 학술논문

目的：建立HPLC法测定鞣酸蛋白原料及制剂中鞣酸的含量。方法：使用Techmate C18-ST（4.6 mm ×250 mm，5μm）色谱柱；流动相A为0.5％冰醋酸溶液，流动相B为乙腈，流量0.8 mL· min-1，梯度洗脱；柱温30.0℃；检测波长272 nm；进样量10μL；使用SPSS19.0软件设计正交试验，最优前处理条件为加入6 mol· L-1盐酸溶液10 mL，5％乙醇5 mL，105℃水解6 h。结果：线性范围为0.001～0.3 mg· mL-1（r＝0.9999），平均加样回收率为97.0％，RSD＝0.5％（n＝9）。结论：本方法简便、准确、专属性强，可用于测定鞣酸蛋白原料及制剂中鞣酸的含量。
목적：건립HPLC법측정유산단백원료급제제중유산적함량。방법：사용Techmate C18-ST（4.6 mm ×250 mm，5μm）색보주；류동상A위0.5％빙작산용액，류동상B위을정，류량0.8 mL· min-1，제도세탈；주온30.0℃；검측파장272 nm；진양량10μL；사용SPSS19.0연건설계정교시험，최우전처리조건위가입6 mol· L-1염산용액10 mL，5％을순5 mL，105℃수해6 h。결과：선성범위위0.001～0.3 mg· mL-1（r＝0.9999），평균가양회수솔위97.0％，RSD＝0.5％（n＝9）。결론：본방법간편、준학、전속성강，가용우측정유산단백원료급제제중유산적함량。
Objective:To establish an HPLC method for the determination of tannic acid in albumin tannate and its preparations.Meth-ods:The column was Techmate C18-ST (4.6 mm ×250 mm, 5μm).The mobile phase A was 0.5%acetic acid and the mobile phase B was acetonitrile ,the flow rate was 0.8 mL· min-1 with gradient elution;The column temperature was 30.0℃and the injection vol-umn was 10μL with a detection wavelength at 272 nm.Orthogonal test was designed by SPSS 19.0 software and results showed that the optimal pretreatment conditions was to join 10 ml of 6 mol· L-1 hydrochloric acid solution and 5 ml of 5%ethanol, hydrolyzing for 6 hours at 105℃.Results: The linear range was 0.001-0.3 mg · mL-1 ( r=0.9999 ) , the average recovery rate was 97.0% and RSD=0.5(n=9).Conclusion: The method is simple, accurate and suitable for determining the content of tannic acid in albumin tannate and its preparations.