中国中西医结合耳鼻咽喉科杂志
中國中西醫結閤耳鼻嚥喉科雜誌
중국중서의결합이비인후과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2014年
2期
85-87,94
,共4页
戴翥%姜翠菊%王梦格%刘娜%唐晓明
戴翥%薑翠菊%王夢格%劉娜%唐曉明
대저%강취국%왕몽격%류나%당효명
变应性鼻炎%滨蒿内酯%细胞因子%疗效机制
變應性鼻炎%濱蒿內酯%細胞因子%療效機製
변응성비염%빈호내지%세포인자%료효궤제
Allergic rhinitis%Scoparone%Cytokines%Therapeutic mechanism
目的：观察滨蒿内酯（Scoparone）对变应性鼻炎（AR）模型大鼠血清Th1/Th2细胞因子表达的影响。方法32只雄性SD大鼠随机分为4组，即正常对照组（NC组）、变应性鼻炎模型组（模型组）、滨蒿内酯组（Sco组）和地塞米松组（Dex组）。以卵清蛋白致敏和激发方法建立变应性鼻炎模型并予相应治疗。观察大鼠行为学改变及鼻黏膜病理组织学变化，ELISA法检测血清IgE和细胞因子IFN-γ、IL-4及IL-5水平。结果在Sco组动物，其行为学评分（1.93±0.70）明显低于AR模型组（6.69±0.88，P＜0.01），与Dex组（2.06±0.55）和NC组（1.45±0.31）比较差异无统计学意义（P＞0.05）；鼻黏膜炎性反应表现明显轻于AR模型组；血清IFN-γ水平（51.01±5.27）明显高于AR模型组（37.41±4.66），IgE、IL-4和IL-5水平则明显低于AR模型组（均P＜0.01），但与Dex组和NC组比较均P＞0.05。结论滨蒿内酯可能通过调节Th1/Th2细胞因子表达并抑制IgE分泌而对变应性鼻炎产生治疗作用。
目的：觀察濱蒿內酯（Scoparone）對變應性鼻炎（AR）模型大鼠血清Th1/Th2細胞因子錶達的影響。方法32隻雄性SD大鼠隨機分為4組，即正常對照組（NC組）、變應性鼻炎模型組（模型組）、濱蒿內酯組（Sco組）和地塞米鬆組（Dex組）。以卵清蛋白緻敏和激髮方法建立變應性鼻炎模型併予相應治療。觀察大鼠行為學改變及鼻黏膜病理組織學變化，ELISA法檢測血清IgE和細胞因子IFN-γ、IL-4及IL-5水平。結果在Sco組動物，其行為學評分（1.93±0.70）明顯低于AR模型組（6.69±0.88，P＜0.01），與Dex組（2.06±0.55）和NC組（1.45±0.31）比較差異無統計學意義（P＞0.05）；鼻黏膜炎性反應錶現明顯輕于AR模型組；血清IFN-γ水平（51.01±5.27）明顯高于AR模型組（37.41±4.66），IgE、IL-4和IL-5水平則明顯低于AR模型組（均P＜0.01），但與Dex組和NC組比較均P＞0.05。結論濱蒿內酯可能通過調節Th1/Th2細胞因子錶達併抑製IgE分泌而對變應性鼻炎產生治療作用。
목적：관찰빈호내지（Scoparone）대변응성비염（AR）모형대서혈청Th1/Th2세포인자표체적영향。방법32지웅성SD대서수궤분위4조，즉정상대조조（NC조）、변응성비염모형조（모형조）、빈호내지조（Sco조）화지새미송조（Dex조）。이란청단백치민화격발방법건립변응성비염모형병여상응치료。관찰대서행위학개변급비점막병리조직학변화，ELISA법검측혈청IgE화세포인자IFN-γ、IL-4급IL-5수평。결과재Sco조동물，기행위학평분（1.93±0.70）명현저우AR모형조（6.69±0.88，P＜0.01），여Dex조（2.06±0.55）화NC조（1.45±0.31）비교차이무통계학의의（P＞0.05）；비점막염성반응표현명현경우AR모형조；혈청IFN-γ수평（51.01±5.27）명현고우AR모형조（37.41±4.66），IgE、IL-4화IL-5수평칙명현저우AR모형조（균P＜0.01），단여Dex조화NC조비교균P＞0.05。결론빈호내지가능통과조절Th1/Th2세포인자표체병억제IgE분비이대변응성비염산생치료작용。
Objective To investigate the effects of Scoparone (Sco) on serum cytokine levels of Th1/Th2 in rats with experimental allergic rhinitis (AR).Methods Thirty two male SD rats were randomly divided into 4 groups, i.e. normal control group (NCG), modeling group (MG), Sco treating group (STG) and dexamethasone treating group (DTG). AR animal models were prepared by the procedures of OVA sensitizing and provocating among SD rats and treated with responsible measures. Then, changes in their behaviors were observed daily and histopathological features of nasal mucosa were evaluated under microscope. The levels of serum IgE and cytokines IFN-γ, IL-4 and IL-5 were determined by the methods ELISA.Results Among the animals of STG, the averaged behavior score was 1.93±0.70, significantly lower than that of MG (6.69±0.88,P<0.01), while there were no obvious statistical significance when compared with that either DTG or NCG (P>0.05). The inflammatory change in nasal mucosa was much reduced in STG as compared with that in MG. The serum level of IFN-γ was 51.01±5.27 in STG, significantly higher than that in MG (37.41±4.66,P<0.01), with its levels of IgE, IL-4 and IL-5 much lower than those of MG (P<0.01) in contrast, while there were no significantly statistical significance when compared with that of NCG or DCG (P>0.05).Conclusions Scoparone is highly effective in treating allergic rhinitis, and this therapeutic effect may be brought about through regulating the expression of Th1/Th2 cytokines to inhibit secretion of IgE.
