CAJ | 학술논문

目的：探讨建立肠道多重耐药细菌感染动物模型的方法，为实验研究和治疗因耐药菌引起的感染提供良好的模型。方法24只6周龄SPF级雄性BALB /c小鼠分成4组：正常对照组、MDR-PA组、MDR-PA＋抗生素组和MDR-PA＋禁食组，6只/组。分别给予生理盐水灌胃、PA悬液灌胃、自由饮用含头孢曲松钠水后PA悬液灌胃和禁食后PA悬液灌胃。实验结束后对结肠组织进行病理学观察和炎症评分；检测炎症因子TNF-α和INF-γ浓度。结果结肠组织病理表现出不同程度的炎症变化；炎症因子TNF-α和INF-γ浓度较对照组均明显增加，有显著统计学意义。结论三种方法均可成功建立肠道多重耐药铜绿假单孢菌感染的动物模型，可根据不同实验目的选择不同的建立模型方法。
목적：탐토건립장도다중내약세균감염동물모형적방법，위실험연구화치료인내약균인기적감염제공량호적모형。방법24지6주령SPF급웅성BALB /c소서분성4조：정상대조조、MDR-PA조、MDR-PA＋항생소조화MDR-PA＋금식조，6지/조。분별급여생리염수관위、PA현액관위、자유음용함두포곡송납수후PA현액관위화금식후PA현액관위。실험결속후대결장조직진행병이학관찰화염증평분；검측염증인자TNF-α화INF-γ농도。결과결장조직병리표현출불동정도적염증변화；염증인자TNF-α화INF-γ농도교대조조균명현증가，유현저통계학의의。결론삼충방법균가성공건립장도다중내약동록가단포균감염적동물모형，가근거불동실험목적선택불동적건립모형방법。
Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria .Methods Male BALB/c mice were divided into four groups:Normal con-trol group, MDR-PA group, MDR-PA +antibiotics group, and MDR-PA +fasting group.They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting , respectively , by ga-vage.Colon tissues were collected for pathological examination and inflammation scoring .The expressions of TNF-αand INF-γin the colon tissue was assessed at the end of the experiment .Results Pathology showed different degrees of inflam-matory changes in the colon tissues .Compared with the normal control group , the expressions of TNF-αand INF-γhad sig-nificant differences with that of experimental groups .Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes .