CAJ | 학술논문

目的：探讨三种不同冷冻保护剂对C57BL/6J小鼠附睾冷冻的效果。方法性成熟并交配过的6～8周龄C57BL/6J雄鼠附睾，分别用3种不同冷冻保护剂二甲基亚砜（DMSO）、丙二醇（PROH）、R18S3进行玻璃化冷冻、复苏和精子采集，观察比较三个实验组的复苏精子形态、存活率以及生殖能力。结果三组冷冻复苏分离后的精子形态完整，具有镰刀头状结构；荧光染色后PROH组精子存活率为（88.17±3.43）％，明显高于DMSO组：（61.17±10.65）％和R18S3组（16.83±6.49）％（ P＜0.05）；经辅助体外受精后均具有受精能力。获得的胚胎移植后可得到正常子代小鼠（DMSO∶PROH∶R18S3＝13∶8∶17）。结论3种冷冻保护剂均适用于C57BL/6J小鼠附睾冷冻，但PROH冷冻效果较好。
목적：탐토삼충불동냉동보호제대C57BL/6J소서부고냉동적효과。방법성성숙병교배과적6～8주령C57BL/6J웅서부고，분별용3충불동냉동보호제이갑기아풍（DMSO）、병이순（PROH）、R18S3진행파리화냉동、복소화정자채집，관찰비교삼개실험조적복소정자형태、존활솔이급생식능력。결과삼조냉동복소분리후적정자형태완정，구유렴도두상결구；형광염색후PROH조정자존활솔위（88.17±3.43）％，명현고우DMSO조：（61.17±10.65）％화R18S3조（16.83±6.49）％（ P＜0.05）；경보조체외수정후균구유수정능력。획득적배태이식후가득도정상자대소서（DMSO∶PROH∶R18S3＝13∶8∶17）。결론3충냉동보호제균괄용우C57BL/6J소서부고냉동，단PROH냉동효과교호。
Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis.Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed , respectively.The morphology of sperm from thawed epididy-mis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants .Results The sperms from thawed epididymis of the three groups were all well-preserved structural-ly.The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solu-tions, respectively, and there were significant differences between the three cryopreservation groups (P<0.05 for all). The number of pups from the DMSO , PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation , respectively.Conclusions All the three cryoprotectants DMSO , PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis.But PROH is the preference of these cryoprotectant solutions .