CAJ | 학술논문

目的：和方法稀有鮈鲫（ Gobiocypris rarus）作为一种本土特有的小型鱼类，是我国正在标准化的化学品毒性测试生物之一。为评价稀有鮈鲫鱼类急性毒性实验中效应的稳定性和可重复性，研究优化筛选了重铬酸钾和3，4-二氯苯胺两种化学品，分别在单一实验室内及不同实验室间进行急性毒性验证实验。结果对于来源相同、体长固定的稀有鮈鲫，无论在实验室内还是实验室间，两组化学品的96 h LC50值均在x珋±2s的质量控制范围之内，所有结果体现了很好的稳定性和可重复性。结论根据研究获得的数据可建立一个有效的稳定性和可重复性衡量数据库，用来评估之后其他单个实验的可靠性；稀有鮈鲫作为一种具有潜力的生态毒性测试的生物种类，具有成为标准实验动物的潜能。
목적：화방법희유구즉（ Gobiocypris rarus）작위일충본토특유적소형어류，시아국정재표준화적화학품독성측시생물지일。위평개희유구즉어류급성독성실험중효응적은정성화가중복성，연구우화사선료중락산갑화3，4-이록분알량충화학품，분별재단일실험실내급불동실험실간진행급성독성험증실험。결과대우래원상동、체장고정적희유구즉，무론재실험실내환시실험실간，량조화학품적96 h LC50치균재x류±2s적질량공제범위지내，소유결과체현료흔호적은정성화가중복성。결론근거연구획득적수거가건립일개유효적은정성화가중복성형량수거고，용래평고지후기타단개실험적가고성；희유구즉작위일충구유잠력적생태독성측시적생물충류，구유성위표준실험동물적잠능。
Objective and Methods As a local species, Chinese rare minnow (Gobiocypris rarus) has been one of the standardized test fish for chemical toxicity tests in China .By optimal screening , the acute toxicity of potassium bichromate and 3,4-dichloroaniline (3,4-DCA) was determined to evaluate repeatability and accuracy for Gobiocypris rarus in one laboratory and between different laboratories .Result Based on a proper data analysis , for the two chemicals per-formed with the same fish, all 96 h LC50 values were within mean ( x-) and upper and lower control limits ( ±2s) in both inner test ( in one laboratory ) and outer test ( between different laboratories ) .Conclusions From these results , a valid database could also be established to evaluate one single test .In addition, Gobiocypris rarus will not only be a potential test species for ecotoxicity tests , but also recommended as a standard laboratory animal .