CAJ | 학술논문

目的：探讨改良半椎板切除法建立大鼠腰神经根压迫模型的优势和特点。方法选用SD大鼠40只，随机分为实验组和对照组，实验组采用改良半椎板切除法建立大鼠腰神经根压迫模型，对照组则采用全椎板切除法，通过观察两组建模手术时间、术中出血量、伤口愈合情况、死亡率、大鼠下肢神经功能、神经根组织病理改变及TNF-α及IL-1在细胞质中灰度值表达水平评估两种方法的效果。结果实验组在建模手术时间、术中出血量、伤口愈合状况、死亡率明显少于对照组（P＜0.01），而大鼠下肢神经功能、神经根组织病理改变及TNF-α及IL-1在细胞质中灰度值表达水平无明显差异，同时，实验组所需的切口小，脊柱后方软组织破坏少。结论采用改良半椎板切除法可保证成功建立大鼠腰神经根压迫模型，并且这一改良方法具有手术时间短，伤口愈合快，出血量少，软组织破坏少，死亡率低等优点，这一改良方法更注重动物伦理。
목적：탐토개량반추판절제법건립대서요신경근압박모형적우세화특점。방법선용SD대서40지，수궤분위실험조화대조조，실험조채용개량반추판절제법건립대서요신경근압박모형，대조조칙채용전추판절제법，통과관찰량조건모수술시간、술중출혈량、상구유합정황、사망솔、대서하지신경공능、신경근조직병리개변급TNF-α급IL-1재세포질중회도치표체수평평고량충방법적효과。결과실험조재건모수술시간、술중출혈량、상구유합상황、사망솔명현소우대조조（P＜0.01），이대서하지신경공능、신경근조직병리개변급TNF-α급IL-1재세포질중회도치표체수평무명현차이，동시，실험조소수적절구소，척주후방연조직파배소。결론채용개량반추판절제법가보증성공건립대서요신경근압박모형，병차저일개량방법구유수술시간단，상구유합쾌，출혈량소，연조직파배소，사망솔저등우점，저일개량방법경주중동물윤리。
Objective To explore the characteristics and advantages of the rat model of lumbar nerve root com -pression established via modified hemilaminectomy approach .Methods Forty Sprague Dawley ( SD) rats were randomly divided into two groups ( experimental group and control group ) for establishing a rat model of lumbar nerve root compres-sion.Modified hemilaminectomy apprpoach was used in the experimental group , while total laminectomy was applied in the control group.Duration of operation, estimated blood loss, wound healing status, postoperative mortality, motor function of the lower extremities , pathological changes and the gray level of cytoplasmic TNF-αand IL-1 expression were observed to e-valuate the characteristics and reliability of the two operation approaches in establishing a rat model of lumbar nerve root compression.Results Compared with the control group , duration of operation , estimated blood loss , wound healing sta-tus, postoperative mortality were significantly better in the experimental group (P<0.01), whereas there was non-signifi-cant difference between two groups with regard to motor function of the lower extremities , pathological changes and gray lev-el of the cytoplasmic expression of TNF-αand IL-1 between the two groups .Moreover, the soft tissue structural alterations were reduced in the experimental group , which might be helpful for the rat postoperative rehabilitation .Conclusions Modified hemilaminectomy approach is a reliable alternative method for establishing a rat model of lumbar nerve root com -pression, which is beneficial to shorten the operative time , improve wound healing status , reduce estimated blood loss and damages of soft tissue , and decrease mortality rate .Moreover , this modified method is more consistent with the principles of animal ethics .