CAJ | 학술논문

目的：探讨Olig2在cuprizone 诱导的急性脱髓鞘动物模型中的表达变化规律。方法应用含0.2％cuprizone 饲料饲育小鼠，通过调控饲育时间，造成神经脱髓鞘及髓鞘再生，使用免疫荧光染色和实时定量PCR （ qRT-PCR）的方法，观察模型髓鞘脱失后及髓鞘再生2周后Olig2、少突胶质细胞碱性髓鞘蛋白（ MBP）及星形胶质细胞神经胶质酸性蛋白（ GFAP）的表达变化。结果 Cuprizone 饲育6周后，动物胼胝体白质内髓鞘脱失严重，在恢复正常饲料后，髓鞘逐渐恢复正常结构。正常小鼠大脑Olig2低水平表达。髓鞘脱失后Olig2、GFAP表达增高，并可见Olig2＋/GFAP＋细胞，MBP表达明显降低。髓鞘再生2周后 Olig2表达降低，MBP、GFAP表达增高。结论 Olig2基因在cuprizone诱导的脱髓鞘模型中的表达变化，提示 Olig2可能参与祖细胞向有活性的星形胶质细胞的分化过程，并与胶质瘢痕的形成有关。
목적：탐토Olig2재cuprizone 유도적급성탈수초동물모형중적표체변화규률。방법응용함0.2％cuprizone 사료사육소서，통과조공사육시간，조성신경탈수초급수초재생，사용면역형광염색화실시정량PCR （ qRT-PCR）적방법，관찰모형수초탈실후급수초재생2주후Olig2、소돌효질세포감성수초단백（ MBP）급성형효질세포신경효질산성단백（ GFAP）적표체변화。결과 Cuprizone 사육6주후，동물변지체백질내수초탈실엄중，재회복정상사료후，수초축점회복정상결구。정상소서대뇌Olig2저수평표체。수초탈실후Olig2、GFAP표체증고，병가견Olig2＋/GFAP＋세포，MBP표체명현강저。수초재생2주후 Olig2표체강저，MBP、GFAP표체증고。결론 Olig2기인재cuprizone유도적탈수초모형중적표체변화，제시 Olig2가능삼여조세포향유활성적성형효질세포적분화과정，병여효질반흔적형성유관。
Objective To investigate the expression pattern of transcription factor Olig 2 in cuprizone-induced mouse model of acute demyelination .Methods C57BL/6 mice were fed with 0.2%cuprizone to induce acute demyelina-tion.Immunofluorescence and qRT-PCR were used, and Olig2, MBP and GFAP were detected in the brain tissues of con-trol group and cuprizone-treated groups for 6 weeks and recovery for 2 weeks.Results Severe demyelination occurred in the corpus callosum following 6-weeks exposure to cuprizone , while remyelination was detected in the white matter after the mice were given diet without cuprizone .In the normal mice , Olig2 was expressed in a low level , while the experessions of Olig2 and GFAP were significantly increased , and Olig2 +/GFAP+cells were detected after demyelination .But the expres-sion of MBP was below the normal level with demyelination .After recovery for 2 weeks, the experession of Olig2 was lower, but the experessions of MBP and GFAP were increased .Conclusions Olig2 may play an important role in the glial differ-entiation from neural progenitor cells into active astrocytes , and in the glial scar formation .