CAJ | 학술논문

目的：探索大小承气汤为基础的多因素大鼠脾虚模型和脾虚肝癌模型的制作方法和成模差别。方法采用苦寒泻下、寒湿环境、劳累、隔天禁食的方法制作脾虚大鼠模型，其中苦寒泻下因素采用大承气汤和小承气汤分别干预；Walker256大鼠肝癌细胞经裸鼠皮下增殖后移植入大鼠肝脏，制作肝癌模型。大鼠随机分为正常组、空白肝癌组、大承气汤组、小承气汤组，每组15只3周龄Wistar雄性大鼠，脾虚因素干预30 d后恢复7d制作肝癌模型并观察35 d。实验过程中观察动物脾虚程度、体重变化、成瘤情况、生存时间等。结果大、小承气汤组动物在脾虚造模过程中相对于对照组（P＜0.01），体重增长受到明显抑制，脾虚造模前20 d大承气汤组动物体重均高于小承气汤组（P＜0.05），之后二者无差别（P＞0.05）。大、小承气汤组动物平均脾虚积分高于空白肝癌组，小承气汤组最高（ P＜0.01）。肝癌模型总成瘤率91.1％，空白肝癌组为80％，大、小承气汤组均为93.3％。小承气汤组大鼠平均生存天数小于肝癌组和大承气汤组（ P＜0.01和0.05）。生存分析提示脾虚积分高的肝癌模型和小承气汤组肝癌模型的生存能力明显下降（ P＜0.05）。结论小承气汤在多因素制作脾虚模型过程中致脾虚作用比大承气汤强，脾虚明显是肝癌模型预后不良的重要因素。
목적：탐색대소승기탕위기출적다인소대서비허모형화비허간암모형적제작방법화성모차별。방법채용고한사하、한습배경、로루、격천금식적방법제작비허대서모형，기중고한사하인소채용대승기탕화소승기탕분별간예；Walker256대서간암세포경라서피하증식후이식입대서간장，제작간암모형。대서수궤분위정상조、공백간암조、대승기탕조、소승기탕조，매조15지3주령Wistar웅성대서，비허인소간예30 d후회복7d제작간암모형병관찰35 d。실험과정중관찰동물비허정도、체중변화、성류정황、생존시간등。결과대、소승기탕조동물재비허조모과정중상대우대조조（P＜0.01），체중증장수도명현억제，비허조모전20 d대승기탕조동물체중균고우소승기탕조（P＜0.05），지후이자무차별（P＞0.05）。대、소승기탕조동물평균비허적분고우공백간암조，소승기탕조최고（ P＜0.01）。간암모형총성류솔91.1％，공백간암조위80％，대、소승기탕조균위93.3％。소승기탕조대서평균생존천수소우간암조화대승기탕조（ P＜0.01화0.05）。생존분석제시비허적분고적간암모형화소승기탕조간암모형적생존능력명현하강（ P＜0.05）。결론소승기탕재다인소제작비허모형과정중치비허작용비대승기탕강，비허명현시간암모형예후불량적중요인소。
Objective To explore the establishing methods and differences of rat models of spleen deficiency and spleen deficiency liver cancer using the traditional Chinese medicine Dachengqi and Xiaochengqi decoctions .Methods Spleen-deficiency rat models were developed by multifactor methods:bitter-cold purgation ( Dachengqi or Xiaochengqi de-coction), cold-wet environment, tiredness, and fasting on alternate days for 30 days.Seven days after spleen-deficiency modeled,liver cancer in the spleen-deficiency rats and normal rats was developed by subcutaneously inoculation of Walker -256 carcinoma cell line in nude mice and then transplanted into rat livers .Liver cancer models were observed for 35 days. Sixty 3-week old male Wistar rats were randomly distributed into 4 groups: normal group , liver cancer model group , and Dachengqi and Xiaochengqi decoction groups .Degree of spleen deficiency , changes of the body-weight, survival time and tumor formation were recorded .Results Spleen deficiency rat models were successfully established .The weight gain of rats in the spleen-deficiency groups was significantly inhibited (P<0.01), and during the first 20 days (but not later) the average body weight of the Dachengqi decoction group was significantly higher than that of the Xiaochengqi decoction group (P<0.05).Spleen-deficiency scores of rats in the Xiaochengqi and Dachengqi groups were higher than those in the blank tumor group, especially in the Xiaochengqi group (P<0.01).The total tumor formation rate was 91.1%and 80%in the blank tumor groups , and 93.3%in both Xiaochengqi and Dachengqi groups , respectively .The average survival time of Xi-aochengqi group was lower than that of the blank tumor and Dachengqi groups ( P<0.01 and P<0.05 ) .The cumulative survival rate of the Xiaochengqi group and rats with a higher spleen-deficiency score was lower than that of the other groups (P<0.05).Conclusions Xiaochengqi decoction may induce spleen deficiency more seriously than Dachengqi decoction , and spleen deficiency may be an important unfavorable prognostic factor for rat models of liver cancer .