CAJ | 학술논문

目的：评估近红外荧光染料IR-783在犬自发性肿瘤中的特异性成像。方法将IR-783染料（5μmol/kg）腹腔注射荷瘤裸鼠，通过活体成像仪检测IR-783的代谢周期，在此基础上，将IR-783染料（1.5μmol/kg）通过后肢静脉注射入自发肿瘤犬体内，5 d后手术切除肿瘤组织，分别进行荧光成像、组织切片HE染色、冰冻切片荧光观察。结果 IR-783染料注射荷瘤裸鼠后可以在肿瘤部位检测到特异性荧光，连续观察8 d，仍有较强的荧光。 IR-783注射自发肿瘤犬5 d后，可在肿瘤组织中检测到特异性荧光。结论近红外荧光染料IR-783能够被肿瘤组织特异性吸收，可用于犬自发性肿瘤的特异性诊断，具有重要的临床应用前景。
목적：평고근홍외형광염료IR-783재견자발성종류중적특이성성상。방법장IR-783염료（5μmol/kg）복강주사하류라서，통과활체성상의검측IR-783적대사주기，재차기출상，장IR-783염료（1.5μmol/kg）통과후지정맥주사입자발종류견체내，5 d후수술절제종류조직，분별진행형광성상、조직절편HE염색、빙동절편형광관찰。결과 IR-783염료주사하류라서후가이재종류부위검측도특이성형광，련속관찰8 d，잉유교강적형광。 IR-783주사자발종류견5 d후，가재종류조직중검측도특이성형광。결론근홍외형광염료IR-783능구피종류조직특이성흡수，가용우견자발성종류적특이성진단，구유중요적림상응용전경。
Objective To evaluate the effect of near infrared heptamethine cyanine dye IR-783-mediated specific tumor imaging in spontaneous tumor of dogs .Methods IR-783 was intraperitoneally injected to nude mice models of transplanted tumor in a dose of 5μmol/kg.The metabolic time course of IR-783 was detected by in vivo imager .Based on the results of above observation , IR-783 was injected to dogs with spontaneous tumor in a dose of 1.5μmol/kg.The site of intravenous injection was the hind leg .Tumor and peri-tumoral tissues were removed at 5 days after IR-783-injection for fluorescence imaging , pathology and frozen section fluorescence examinations .Results After i.p.IR-783 injection to nude mice models of transplanted tumor , the transplanted tumor tissues of nude mice had stronger specific fluorescence than normal tissues by imaging at 8 days after injection .After i.v.IR-783 injection to four dogs with spontaneous tumor , the fluorescence signal in the tumor tissues was stronger than that in the normal tissues at 5 days after injection .Conclusions Near infrared fluorescent dye IR-783 could be specifically taken up by tumor tissues , and can be used for specific diagnosis of tumor.It has an important clinical application prospect .