CAJ | 학술논문

目的：观察不同时间点阿霉素肾病小鼠肾脏病理的转变过程。方法48只雄性 BALB/c小鼠，随机分成对照组和模型组，模型组经尾静脉一次性注射阿霉素10.5mg/kg，对照组给予等量的生理盐水。动态观察实验12周内小鼠24 h尿蛋白、血清生化指标、肾脏病理改变。结果模型小鼠蛋白尿于实验第2周出现，持续至第12周，第8周出现高峰（均P＜0.05）；低蛋白血症、高脂血症分别于实验第4、8周出现，血肌酐于实验第12周明显高于正常组（均 P＜0.05）。模型小鼠肾脏病理改变第4周表现为微小病变型；第8周病变较第4周加重，硬化不明显；第12周出现肾小球局灶节段性硬化、肾小球硬化指数（ GSI）为（2.81±0.84）％，明显高于同一观测时间点对照组 GSI（0.33±0.21）％（ P ＜0.01）。结论一次性尾静脉注射10.5mg/kg阿霉素，能成功复制阿霉素肾病小鼠模型，该模型在早期表现为微小病变型肾病，晚期转变为局灶性节段性肾小球硬化。
목적：관찰불동시간점아매소신병소서신장병리적전변과정。방법48지웅성 BALB/c소서，수궤분성대조조화모형조，모형조경미정맥일차성주사아매소10.5mg/kg，대조조급여등량적생리염수。동태관찰실험12주내소서24 h뇨단백、혈청생화지표、신장병리개변。결과모형소서단백뇨우실험제2주출현，지속지제12주，제8주출현고봉（균P＜0.05）；저단백혈증、고지혈증분별우실험제4、8주출현，혈기항우실험제12주명현고우정상조（균 P＜0.05）。모형소서신장병리개변제4주표현위미소병변형；제8주병변교제4주가중，경화불명현；제12주출현신소구국조절단성경화、신소구경화지수（ GSI）위（2.81±0.84）％，명현고우동일관측시간점대조조 GSI（0.33±0.21）％（ P ＜0.01）。결론일차성미정맥주사10.5mg/kg아매소，능성공복제아매소신병소서모형，해모형재조기표현위미소병변형신병，만기전변위국조성절단성신소구경화。
Objective Our purpose was to observe the renal pathological changes in the mouse modells of adriamy -cin-induced nephropathy in different periods .Method 48 healthy male BALB/c mice were randomly divided into control group and model group .The model group received a disposable tail vein injection of adriamycin 10.5 mg/kg body weight , and the control group received the same amount of saline .24-hour urinary protein , serum biochemical indexes and kidney pathological changes were dynamically observed for 12 weeks.Results Proteinuria of model mice appeared in the 2th week after ADR injection, which lasted to the end of the 12-week experiment, At the 8th week, the amount of urine protein reached a peak (P<0.05);The serum albumin was decreased at the 4th week, cholesterol was increased at 8th week.At the end of experiment, serum creatinine was also increased (P<0.05).Minimal change nephrotic syndrome (MCNS) was observed in model mice at the 4th week;the lesions in renal tissues at 8th weeks were more serious than that at 4th weeks, but glomerular sclerosis was unconspicuous .Focal segmental glomerulonephritis ( FSGS) was seen at the 12th week.The GSI of the model mice was(2.81 ±0.84)%, significantly higher than that of the control mice ((0.33 ±0.21)%) at 12th week(P<0.01).Conclusions A mouse model with adriamycin-induced-nephrosis can be successfully established by a disposable tail vein injection of adriamycin in a dose of 10.5 mg/kg body weight .The early manifest ation of this model is MCNS, and at a late stage , it may be changed into FSGS .