CAJ | 학술논문

目的：研究骨髓间充质干细胞（Bone Marrow Mesenchymal Stem Cells，BMMSCs）对小胶质细胞介导的炎症反应的抑制作用。方法实验分为四组：组一：小胶质细胞（BV2）生长于DMEM（High Glucose）培养液中；组二：BV2细胞生长于加入脂多糖（ LPS）的上述培养液中；组三：BV2细胞、BMMSCs共培养于加入LPS的上述培养液中；组四：骨髓间充质干细胞（ BMMSCs ）生长于加入LPS的上述培养液中。观察BV2细胞的生长状态、电镜超微结构变化及其分泌的炎症因子TNF-α表达量的变化。结果光镜下BV2细胞密度依次为：组一＞组三＞组二，组四中BMMSCs生长状态良好；电镜下可见组二BV2细胞内出现大量肿胀及空泡化的线粒体、内质网等细胞器，少见生长活跃多核仁细胞，同时可见大量崩解细胞，组三细胞状态明显好于组二；BV2细胞分泌的炎症因子TNF-α表达量依次为组二＞组三＞组一＞组四。结论 BM-MSCs抑制小胶质细胞介导的炎症反应，进而发挥神经保护作用。
목적：연구골수간충질간세포（Bone Marrow Mesenchymal Stem Cells，BMMSCs）대소효질세포개도적염증반응적억제작용。방법실험분위사조：조일：소효질세포（BV2）생장우DMEM（High Glucose）배양액중；조이：BV2세포생장우가입지다당（ LPS）적상술배양액중；조삼：BV2세포、BMMSCs공배양우가입LPS적상술배양액중；조사：골수간충질간세포（ BMMSCs ）생장우가입LPS적상술배양액중。관찰BV2세포적생장상태、전경초미결구변화급기분비적염증인자TNF-α표체량적변화。결과광경하BV2세포밀도의차위：조일＞조삼＞조이，조사중BMMSCs생장상태량호；전경하가견조이BV2세포내출현대량종창급공포화적선립체、내질망등세포기，소견생장활약다핵인세포，동시가견대량붕해세포，조삼세포상태명현호우조이；BV2세포분비적염증인자TNF-α표체량의차위조이＞조삼＞조일＞조사。결론 BM-MSCs억제소효질세포개도적염증반응，진이발휘신경보호작용。
Objective To investigate the inhibitory effect of bone marrow mesenchymal stem cells ( BMMSC) on the inflammatory response of microglial cells .Methods The samples were divided into four groups .Group I:microglial (BV-2) cells were cultured in DMEM (high glucose).Group II: BV-2 cells were cultured in DMEM containing lipopo-lysaccharide (LPS).Group III:BV-2cells and BMMSCs were co-cultured in DMEM.Group IV: BMMSCs were cultured in DMEM containing LPS .The growth state and ultrastructure of BV-2 cells were observed and the changes of TNF-αex-pression were detected .Results Different cell densities of BV-2 cells were observed under the optical microscope in an or-der from high level to low level:group I >group III >group II.The expressions of TNF-αwere:group Ⅱ >groupⅢ>group Ⅰ.Ultrastructural observation of BV-2 cells showed that there were a large number of swollen mitochondria and endoplasmic reticulum , some of them showed vacuolization .No BV-2 cells with multiple hucleoli were found in the group II indicating the absence of active cell growth .At the same time, cytolysis was observed only in the group II .The growth of BV-2 cells in the group III was better than that in the group II .Conclusions BMMSCs can inhibit inflammatory response of microglial cells, therefore, play a neuroprotective role.