CAJ | 학술논문

利用湖北省四个地点的马铃薯青枯病样本，对其病原物的菌落形态、碳水化合物利用、显微形态、鞭毛引物序列、演化型以及致病力等方面进行了分析。结果显示，在2,3,5-氯化三苯基四氮唑培养基上检测得到16个病原物菌落符合青枯菌菌落形态特征，包括菌落圆形，隆起，中间红色，乳白色分泌物，并伴有褐色物质；显微观察16个病原物中仅有6个病原物具有短杆状、两端钝圆的特点；而其中能够通过PCR扩增得到鞭毛序列的病原物仅有源自武汉的HZ4-14和HZ5-1，序列比对发现两者序列一致且与GMI1000等已测序青枯菌株同源性达到99%；演化型测定结果显示HZ4-14和HZ5-1均能得到144 bp的目标条带，为演化II型；利用四种不同马铃薯材料试管苗伤根接种鉴定结果显示两个菌株均具有一定致病力。结果表明，引起湖北省武汉市青枯病的病原物为雷尔氏青枯菌Ralstonia solanacearum，生理小种2号，演化II型（美洲组）。
이용호북성사개지점적마령서청고병양본，대기병원물적균락형태、탄수화합물이용、현미형태、편모인물서렬、연화형이급치병력등방면진행료분석。결과현시，재2,3,5-록화삼분기사담서배양기상검측득도16개병원물균락부합청고균균락형태특정，포괄균락원형，륭기，중간홍색，유백색분비물，병반유갈색물질；현미관찰16개병원물중부유6개병원물구유단간상、량단둔원적특점；이기중능구통과PCR확증득도편모서렬적병원물부유원자무한적HZ4-14화HZ5-1，서렬비대발현량자서렬일치차여GMI1000등이측서청고균주동원성체도99%；연화형측정결과현시HZ4-14화HZ5-1균능득도144 bp적목표조대，위연화II형；이용사충불동마령서재료시관묘상근접충감정결과현시량개균주균구유일정치병력。결과표명，인기호북성무한시청고병적병원물위뢰이씨청고균Ralstonia solanacearum，생리소충2호，연화II형（미주조）。
The colony morphology, carbohydrate utilization, microscopy, fliC sequence, phylotype and pathogenicity of Ralstonia solanacearum were studied by using the diseased potato from four cities of Hubei Province in order to investigate the etiology of the disease bacterial wilt. Sixteen strains exhibited irregular round, upheaval, pink colonies with white margin differential y on BG medium with 2,3,5-triphenyl tetrazolium chloride. Six strains of them showed the targeted morphology of R. solanacearum by microscope. Only two strains had targeted fragments, which were amplified by the specific fliC primers. The targeted fragments were sequenced. The results revealed that HZ4-14 and HZ5-1 had 99%nucleotide matching with the fliC gene of R. solanacearum GMI1000. The results of phylotype multiple PCR indicated that the specific 144 bp fragments were amplified from two strains. HZ4-14 and HZ5-1 were virulent to potato and showed the typical bacterial wilt symptom. These results demonstrate that the pathogen of bacterial wilt in Wuhan belongs to Bivoar II, Phylotype II of R. solanacearum.