CAJ | 학술논문

丝状藻体是紫菜生活史中的重要阶段，其生长增殖与环境条件密切相关．本研究考察了与坛紫菜丝状藻体生长增殖相关的多个影响因子，探讨了可促进坛紫菜丝状藻体生长、达到生长速率快的优化调控培养条件．具体操作实施过程为：初始丝状藻体来自成熟紫菜叶状体的果孢子释放，基于8因子3水平的正交实验设计方案[采用正交表L27（313）]，确定了丝状藻体生长对多个环境因子的响应以及优化的调控培养条件．结果表明，除开pH、复合维生素浓度和NaHCO3浓度外，其他因子对丝状藻体生长均有显著的影响（p≤0.0438）．获得的优化调控培养条件为：温度18℃、光强27μmol/m2·s-1、盐度25、pH值8.5、复合维生素浓度15μg/dm3、NaHCO3浓度2 mg/dm3、每天光照时数18 h、营养液浓度为f培养介质．优化调控培养条件下的丝状藻体生长速率高达12.07％，平均值±标准偏差SD为（10.70±0.88）％/d．
사상조체시자채생활사중적중요계단，기생장증식여배경조건밀절상관．본연구고찰료여단자채사상조체생장증식상관적다개영향인자，탐토료가촉진단자채사상조체생장、체도생장속솔쾌적우화조공배양조건．구체조작실시과정위：초시사상조체래자성숙자채협상체적과포자석방，기우8인자3수평적정교실험설계방안[채용정교표L27（313）]，학정료사상조체생장대다개배경인자적향응이급우화적조공배양조건．결과표명，제개pH、복합유생소농도화NaHCO3농도외，기타인자대사상조체생장균유현저적영향（p≤0.0438）．획득적우화조공배양조건위：온도18℃、광강27μmol/m2·s-1、염도25、pH치8.5、복합유생소농도15μg/dm3、NaHCO3농도2 mg/dm3、매천광조시수18 h、영양액농도위f배양개질．우화조공배양조건하적사상조체생장속솔고체12.07％，평균치±표준편차SD위（10.70±0.88）％/d．
The conchocelis is an important phase of Porphyra life cycle and its growth is considerably related to the ambient conditions.We investigated multiple influential factors related to growth and multiplication of Porphyra hai-tanensis conchocelis and studied the optimal controlled culture condition which could promote the conchocelis growth rate.Specific operational processes were taken as initial conchocelis was obtained from carpospores release of mature Porphyra thallus and responses of conchocelis growth to multiple environmental factors and optimal con-trolled culture conditions were determined based on the designation of an orthogonal experimentation with factors and levels[L27(313)].Results demonstrated that all other factors had significant effects on conchocelis growth(p≤0.043 8)except pH,compound vitamin and dicarbonate concentrations.The optimal controlled culture conditions was temperature 18℃,light intensity 27 μmol photons/m2 ·s-1,salinity 25,pH 8.5,compound vitamin 15μg/dm3,dicarbonate 2 mg/dm3,light hour 18 and f nutrition concentration.Under optimal controlled culture con-ditions,conchocelis growth rate was as high as 12.07 %/d with average ±SD:(10.70 ±0.88)%/d.