CAJ | 학술논문

反硝化与厌氧氨氧化作用是海洋结合态氮迁出的主要途径，15 N示踪法是定量反硝化和厌氧氨氧化作用的新方法，它具有灵敏度高、可同步测量反硝化与厌氧氨氧化速率的优点．本研究开展了一系列方法学实验，以确定高纯氦气驱赶水样所含气体的时间、超声赶气时间、同位素比值质谱仪测量N2含量的精度、N2含量及其同位素组成测定的工作曲线以及示踪剂的最佳添加量等，从而确立起15 N示踪同步测定海水反硝化和厌氧氨氧化速率的方法．所建立方法测量N2含量的相对标准偏差小于8％，可满足海洋反硝化与厌氧氨氧化速率测定的需要．应用该方法实测了厦门！"湖富氧与缺氧水体的反硝化与厌氧氨氧化速率，结果表明，缺氧底层水的反硝化速率[4.10～5.47μmol/（dm3·d）]比富氧表层水[0.43～0.46μmol/（dm3·d）]明显来得高，但缺氧底层水的厌氧氨氧化速率[0．00～0.12μmol/（dm3·d）]则比富氧表层水的速率[0.52～0.67μmol/（dm3·d）]低得多．
반초화여염양안양화작용시해양결합태담천출적주요도경，15 N시종법시정량반초화화염양안양화작용적신방법，타구유령민도고、가동보측량반초화여염양안양화속솔적우점．본연구개전료일계렬방법학실험，이학정고순양기구간수양소함기체적시간、초성간기시간、동위소비치질보의측량N2함량적정도、N2함량급기동위소조성측정적공작곡선이급시종제적최가첨가량등，종이학립기15 N시종동보측정해수반초화화염양안양화속솔적방법．소건립방법측량N2함량적상대표준편차소우8％，가만족해양반초화여염양안양화속솔측정적수요．응용해방법실측료하문！"호부양여결양수체적반초화여염양안양화속솔，결과표명，결양저층수적반초화속솔[4.10～5.47μmol/（dm3·d）]비부양표층수[0.43～0.46μmol/（dm3·d）]명현래득고，단결양저층수적염양안양화속솔[0．00～0.12μmol/（dm3·d）]칙비부양표층수적속솔[0.52～0.67μmol/（dm3·d）]저득다．
Denitrification and anammox are two pathways for removal of fixed nitrogen from marine environments.15 N tracer assay was a new method for simultaneously measuring denitrification and anammox rates in seawater with high sensitivity.In this study,a series of experiments were conducted to assess the purge time for removing natural dis-solved N2 ,the blowing time for N2 concentrated in bottle headspace,the measurement accuracy of N2 determina-tion,the standard curve for calculation of N2 content,and the effect of tracer amounts on the measured denitrifica-tion rate.The established method measured N2 content with an accuracy of less than 8%,which met the need for measurements of denitrification and anammox rates.The measured denitrification and anammox rates in the Yundang lagoon,Xiamen,indicated that the denitrification rates in the anoxic bottom water[4.10~5.47 μmol/(dm3 ·d)] were much higher than those in the aerobic surface water[0.43~0.46 μmol/(dm3 ·d)],while the anammox rates in the bottom water[0.00 ~0.12 μmol/(dm3 ·d)]were lower than those in the surface water[0.52 ~0.67μmol/(dm3 ·d)].