CAJ | 학술논문

目的研究前列腺干细胞抗原(PSCA)mRNA表达与前列腺上皮内瘤(PIN)发生恶性变的关系。方法收集前列腺活检诊断为孤立性PIN 患者117例,其中低级别PIN(LGPIN)51例,高级别PIN(HGPIN)66例。通过核酸原位杂交技术检测 PIN及其继发前列腺癌的 PSCA mRNA表达。卡方检验和t检验分析PSCA mRNA在组间的表达差异。单变量和多变量 Logistic回归分析评价PSCA mRNA表达对PIN进展为前列腺癌的风险。结果根据 Gleason评分,在34例继发前列腺癌中,PSCA mRNA表达水平高于相应的PIN且差异有统计学意义(P<0.001)。与 LGPIN 相比, HGPIN的PSCA mRNA表达水平的增高明显且差异有统计学意义(P<0.01)。相比没有发展为前列腺癌的LGPIN和HGPIN,发展为前列腺癌者的PSCA mRNA的表达水平明显增高(P<0.001和P<0.001)。多变量的Logistic回归分析证明仅PSCA mRNA可以分别预测由孤立性LGPIN及孤立性 HGPIN进展为前列腺癌。结论PIN中PSCA mRNA 的表达可以作为其进展为临床前列腺癌的一个重要预测指标,提示PSCA在前列腺肿瘤发生过程中有重要意义。
목적연구전렬선간세포항원(PSCA)mRNA표체여전렬선상피내류(PIN)발생악성변적관계。방법수집전렬선활검진단위고립성PIN 환자117례,기중저급별PIN(LGPIN)51례,고급별PIN(HGPIN)66례。통과핵산원위잡교기술검측 PIN급기계발전렬선암적 PSCA mRNA표체。잡방검험화t검험분석PSCA mRNA재조간적표체차이。단변량화다변량 Logistic회귀분석평개PSCA mRNA표체대PIN진전위전렬선암적풍험。결과근거 Gleason평분,재34례계발전렬선암중,PSCA mRNA표체수평고우상응적PIN차차이유통계학의의(P<0.001)。여 LGPIN 상비, HGPIN적PSCA mRNA표체수평적증고명현차차이유통계학의의(P<0.01)。상비몰유발전위전렬선암적LGPIN화HGPIN,발전위전렬선암자적PSCA mRNA적표체수평명현증고(P<0.001화P<0.001)。다변량적Logistic회귀분석증명부PSCA mRNA가이분별예측유고립성LGPIN급고립성 HGPIN진전위전렬선암。결론PIN중PSCA mRNA 적표체가이작위기진전위림상전렬선암적일개중요예측지표,제시PSCA재전렬선종류발생과정중유중요의의。
Objective To determine whether prostate stem cell antigen (PSCA)mRNA ex-pression was associated with the presence of cancer in prostatic intraepithelial neoplasia (PIN). Methods One hundred seventeen men were diagnosed with isolated PIN on initial prostate biopsy, 51 with low-grade form (LGPIN),and 66 with high-grade form (HGPIN).PSCA mRNA expres-sion in initial PIN and subsequent cancer was examined by in situ hybridization.The differences of the PSCA mRNA expression level between the groups were analyzed by the Chi-square and Student's t-test.Univariate and multivariate logistic regression analyses were performed to evaluate the pre-dictive performance of PSCA mRNA. Results PSCA mRNA expression level in 34 subsequent cancers was statistically increased compared with their paired PIN (P<0.001),with a Gleason's de-pendence.HGPIN showed statistically high PSCA mRNA expression compared with LGPIN (P<0.01).PSCA mRNA expression levels were significantly stronger in the initial isolated LGPIN and i-solated HGPIN with subsequent cancer than those without (P<0.001 and P<0.001,respectively). Multivariate logistic regression analysis demonstrated that only PSCA mRNA was predictive of the onset of subsequent cancer in patients with isolated LGPIN and in those with isolated HGPIN,re-spectively. Conclusions Our data identify PSCA mRNA in initial PIN as a significant predictor of subsequent cancer,suggesting that PSCA play a importance role in prostatic tumorigenesis.