皮肤性病诊疗学杂志
皮膚性病診療學雜誌
피부성병진료학잡지
DIAGNOSIS AND THERAPY JOURNAL OF DERMATO-VENEREOLOGY
2014年
2期
99-103
,共5页
种树彬%兰海梅%赖梅生%曾抗
種樹彬%蘭海梅%賴梅生%曾抗
충수빈%란해매%뢰매생%증항
放射性皮炎%放射性溃疡%大鼠模型%直线加速器
放射性皮炎%放射性潰瘍%大鼠模型%直線加速器
방사성피염%방사성궤양%대서모형%직선가속기
Radioactive dermatitis%Radioactive ulceration%Rat model%Linear accelerator
目的：建立并评价大鼠急性放射性皮炎/溃疡模型。方法：将48只雌性Wistar大鼠随机分成8组，采用直线加速器发射高能X线建立模型，分别以0、5、15、30、35、38、43、50 Gy的剂量单次局部照射各组大鼠双后肢，筛选确定大鼠急性放射性皮炎和急性放射性溃疡的最佳照射剂量，再将46只同样大鼠随机分成模型1组（38 Gy，20只）、模型2组（43 Gy，20只）和空白对照组（6只），分别于照射后第18、60天观察两组模型照射区域皮肤变化情况、存活情况等，通过光镜和电镜观察其皮肤病理学变化，并与对照组比较。结果：当照射剂量为38 Gy时，大鼠皮肤见显著充血及水肿性红斑；光镜下观察表皮结构完整，真皮间质水肿，伴少量淋巴细胞浸润；电镜下观察细胞核异染色质增多，有核固缩现象。当照射剂量为43 Gy时，其皮肤见红斑、糜烂及较多溃疡；光镜下观察其表皮部分坏死，真皮浅层高度水肿，毛细血管管壁变性坏死；电镜下细胞核固缩明显，细胞器明显变性，血管壁变性坏死。结论：直线加速器单次局部照射38 Gy和43 Gy，可分别成功诱导大鼠双后肢发生急性放射性皮炎和急性放射性溃疡。该两种模型能分别反映大鼠急性放射性皮炎和溃疡模型的临床特征和病理特征，可用于实验研究。
目的：建立併評價大鼠急性放射性皮炎/潰瘍模型。方法：將48隻雌性Wistar大鼠隨機分成8組，採用直線加速器髮射高能X線建立模型，分彆以0、5、15、30、35、38、43、50 Gy的劑量單次跼部照射各組大鼠雙後肢，篩選確定大鼠急性放射性皮炎和急性放射性潰瘍的最佳照射劑量，再將46隻同樣大鼠隨機分成模型1組（38 Gy，20隻）、模型2組（43 Gy，20隻）和空白對照組（6隻），分彆于照射後第18、60天觀察兩組模型照射區域皮膚變化情況、存活情況等，通過光鏡和電鏡觀察其皮膚病理學變化，併與對照組比較。結果：噹照射劑量為38 Gy時，大鼠皮膚見顯著充血及水腫性紅斑；光鏡下觀察錶皮結構完整，真皮間質水腫，伴少量淋巴細胞浸潤；電鏡下觀察細胞覈異染色質增多，有覈固縮現象。噹照射劑量為43 Gy時，其皮膚見紅斑、糜爛及較多潰瘍；光鏡下觀察其錶皮部分壞死，真皮淺層高度水腫，毛細血管管壁變性壞死；電鏡下細胞覈固縮明顯，細胞器明顯變性，血管壁變性壞死。結論：直線加速器單次跼部照射38 Gy和43 Gy，可分彆成功誘導大鼠雙後肢髮生急性放射性皮炎和急性放射性潰瘍。該兩種模型能分彆反映大鼠急性放射性皮炎和潰瘍模型的臨床特徵和病理特徵，可用于實驗研究。
목적：건립병평개대서급성방사성피염/궤양모형。방법：장48지자성Wistar대서수궤분성8조，채용직선가속기발사고능X선건립모형，분별이0、5、15、30、35、38、43、50 Gy적제량단차국부조사각조대서쌍후지，사선학정대서급성방사성피염화급성방사성궤양적최가조사제량，재장46지동양대서수궤분성모형1조（38 Gy，20지）、모형2조（43 Gy，20지）화공백대조조（6지），분별우조사후제18、60천관찰량조모형조사구역피부변화정황、존활정황등，통과광경화전경관찰기피부병이학변화，병여대조조비교。결과：당조사제량위38 Gy시，대서피부견현저충혈급수종성홍반；광경하관찰표피결구완정，진피간질수종，반소량림파세포침윤；전경하관찰세포핵이염색질증다，유핵고축현상。당조사제량위43 Gy시，기피부견홍반、미란급교다궤양；광경하관찰기표피부분배사，진피천층고도수종，모세혈관관벽변성배사；전경하세포핵고축명현，세포기명현변성，혈관벽변성배사。결론：직선가속기단차국부조사38 Gy화43 Gy，가분별성공유도대서쌍후지발생급성방사성피염화급성방사성궤양。해량충모형능분별반영대서급성방사성피염화궤양모형적림상특정화병리특정，가용우실험연구。
Objective:To establish the rat models of acute radioactive dermatitis/ulceration. Methods:Forty-eight rats were randomly assigned to eight groups , locally treated on two back legs by different dosage of X-ray radiation respectively at 0-, 5-, 15-, 30-, 35-, 38-, 43-and 50 Gy. After phenotypic analysis of optimal dosages for each model , 46 female Wistar Rats were randomly distributed into 38-Gy group(n=20), 43-Gy group(n=20) and control group(n=6).After 18 days, 60 days, the change of the skin and hair in irradiation area and survival situation of the models were visual assessed , and the pathological changes of the skin were detected with optical microscopy and scanning electron microscopy .Results:The rats irradiated by a 38-Gy X-ray pres-ent marked hyperemia and edematous erythema , and hair loss in the exposure zone .Optical mi-croscopy revealed the integral epidermis structure , interstitial edema , genuine layer were infiltra-ted with a small amount of lymphocytes .SEM showed nucleus heterochromatin and nuclear pykno-sisincreased.After 43-Gy irradation, the skin erythema, erosion and more ulcers were observed . Microscopically , the partial epidermal necrosis , significant edema at the upper dermis , degenera-tion and necrosis of capillary blood vessels , surrounded by a large number of neutrophils , eosino-phils and nuclear dust distribution can be obsvered .Ultra-structure analysis were presented in those fingings such as the nucleus pyknosis , cell organelle degeneration , hemal wall degeneration necrosis.Conclusion:Rat models of acute radioactive dermatitis and acute radioactive ulceration were successfully established by 38-Gy and 43-Gy high-dose X-ray radiation respectively , the ap-pearance of the models in the clinical and histopathological were resembe with the features of acute radioactive dermatitis and ulceration .
