皮肤性病诊疗学杂志
皮膚性病診療學雜誌
피부성병진료학잡지
DIAGNOSIS AND THERAPY JOURNAL OF DERMATO-VENEREOLOGY
2014年
2期
95-98
,共4页
308 nm准分子激光%白癜风%肿瘤坏死因子α%碱性成纤维细胞生长因子
308 nm準分子激光%白癜風%腫瘤壞死因子α%堿性成纖維細胞生長因子
308 nm준분자격광%백전풍%종류배사인자α%감성성섬유세포생장인자
308 nm excimer laser%Vitiligo%TNF-α%bFGF
目的：评价308 nm 准分子激光对寻常型稳定期白癜风患者皮肤组织液肿瘤坏死因子（ TNF-α）、碱性成纤维细胞生长因子（ bFGF）水平的影响。方法：对28例寻常型稳定期白癜风患者进行负压吸疱表皮移植，先将白斑、围白斑、正常皮肤分为非激光区和激光区。在移植前，激光区接受308 nm准分子激光治疗10次；非激光区不作处理。吸疱移植时收集各区疱液，采用酶联免疫吸附法（ ELISA）测定皮肤组织液中TNF-α和bFGF水平。结果：白斑、围白斑、正常皮肤的激光区分别与各自的非激光区比较，皮肤组织液的 TNF-α均有不同程度的降低，而 bFGF升高，差异均有统计学意义（ P值均＜0.05）。白斑、围白斑的非激光区和激光区分别与正常皮肤的非激光区和激光区比较，TNF-α较高，bFGF较低，差异均有统计学意义（ P值均＜0.05）；而白斑非激光区、激光区的TNF-α、bFGF水平分别与围白斑非激光区、激光区比较，差异均无统计学意义（P值均＞0.05）。结论：308 nm准分子激光能下调皮肤组织液内的TNF-α水平，提高bFGF的水平，可能是其治疗白癜风的机制之一。
目的：評價308 nm 準分子激光對尋常型穩定期白癜風患者皮膚組織液腫瘤壞死因子（ TNF-α）、堿性成纖維細胞生長因子（ bFGF）水平的影響。方法：對28例尋常型穩定期白癜風患者進行負壓吸皰錶皮移植，先將白斑、圍白斑、正常皮膚分為非激光區和激光區。在移植前，激光區接受308 nm準分子激光治療10次；非激光區不作處理。吸皰移植時收集各區皰液，採用酶聯免疫吸附法（ ELISA）測定皮膚組織液中TNF-α和bFGF水平。結果：白斑、圍白斑、正常皮膚的激光區分彆與各自的非激光區比較，皮膚組織液的 TNF-α均有不同程度的降低，而 bFGF升高，差異均有統計學意義（ P值均＜0.05）。白斑、圍白斑的非激光區和激光區分彆與正常皮膚的非激光區和激光區比較，TNF-α較高，bFGF較低，差異均有統計學意義（ P值均＜0.05）；而白斑非激光區、激光區的TNF-α、bFGF水平分彆與圍白斑非激光區、激光區比較，差異均無統計學意義（P值均＞0.05）。結論：308 nm準分子激光能下調皮膚組織液內的TNF-α水平，提高bFGF的水平，可能是其治療白癜風的機製之一。
목적：평개308 nm 준분자격광대심상형은정기백전풍환자피부조직액종류배사인자（ TNF-α）、감성성섬유세포생장인자（ bFGF）수평적영향。방법：대28례심상형은정기백전풍환자진행부압흡포표피이식，선장백반、위백반、정상피부분위비격광구화격광구。재이식전，격광구접수308 nm준분자격광치료10차；비격광구불작처리。흡포이식시수집각구포액，채용매련면역흡부법（ ELISA）측정피부조직액중TNF-α화bFGF수평。결과：백반、위백반、정상피부적격광구분별여각자적비격광구비교，피부조직액적 TNF-α균유불동정도적강저，이 bFGF승고，차이균유통계학의의（ P치균＜0.05）。백반、위백반적비격광구화격광구분별여정상피부적비격광구화격광구비교，TNF-α교고，bFGF교저，차이균유통계학의의（ P치균＜0.05）；이백반비격광구、격광구적TNF-α、bFGF수평분별여위백반비격광구、격광구비교，차이균무통계학의의（P치균＞0.05）。결론：308 nm준분자격광능하조피부조직액내적TNF-α수평，제고bFGF적수평，가능시기치료백전풍적궤제지일。
Objective:To evaluate the influence of the 308 nm excimer laser on the level of the tumor necrosis factor-α( TNF-α) and the basic fibroblastic growth factor ( bFGF) in the skin tissue fluid of patients with vitiligo vulgaris in stable stage .Methods:28 patients with vitiligo vulgaris in stable stage receiving autologous epidermal grafting were included in this study .The vitiliginous lesion , the perilesional skin and the normal skin were divided into laser treatment section and non-laser treatment section . Before grafting , the laser treatment section were irradiated with the 308 nm excimer laser for ten times .The suction blister fluid in different section were collected , and the level of TNF-αand bFGF of those fluids in different section were measured by the enzyme linked immunosorbent assay .Results:In vitiliginous lesion , perilesional skin and normal skin , the TNF-αlevel in the laser treatment section were lower than that in the non-laser treatment section , while the bFGF level in the laser treatment section were higher than that in the non-laser treatment section(P<0.05).The TNF-αlevel in the non-laser section in vitiliginous lesion and in perile-sional skin were significantly higher than that in normal skin ( P<0.05 ) , and the bFGF level in non-laser section in vitiliginous lesion and in perilesional skin were significantly lower than that in normal skin(P<0.05).There were no significant difference in the level of TNF-αand bFGF in non-laser section and the laser section between the vitiliginous lesion and the perilesional skin (P>0.05).Conclusion:The 308 nm excimer laser can inhibit the keratinocyte to secret TNF-αand upgrade the level of bFGF in tissue fluids , which would be one of mechanisms of the 308 nm excimer laser in treatment of vitiligo on inducing repigmentation of skin lesions .
