CAJ | 학술논문

为了探讨猴B病毒gG基因编码蛋白作为疫苗候选抗原和ELISA试剂盒研制所需检测用抗原的可能性，本研究从Genbank数据库中获得猴B病毒gG基因序列，采用IEDB、SOSUI等在线分析系统对该基因序列的编码区进行生物信息学分析，并预测gG基因编码蛋白的理化性质、可溶性和表面可及性、跨膜区、抗原表位以及线性B细胞表位等。分析结果表明，B病毒gG蛋白为膜蛋白，具有一个跨膜螺旋，以及较为丰富的β-转角，为可溶性蛋白，蛋白序列中具有多个抗原表位和优势线性B细胞表位，有作为猴B病毒疫苗候选抗原和抗体诊断用抗原的潜力。
위료탐토후B병독gG기인편마단백작위역묘후선항원화ELISA시제합연제소수검측용항원적가능성，본연구종Genbank수거고중획득후B병독gG기인서렬，채용IEDB、SOSUI등재선분석계통대해기인서렬적편마구진행생물신식학분석，병예측gG기인편마단백적이화성질、가용성화표면가급성、과막구、항원표위이급선성B세포표위등。분석결과표명，B병독gG단백위막단백，구유일개과막라선，이급교위봉부적β-전각，위가용성단백，단백서렬중구유다개항원표위화우세선성B세포표위，유작위후B병독역묘후선항원화항체진단용항원적잠력。
To explore the possibility of the monkey B virus gG gene encoded protein as a vaccine candidate anti-gen and detecting antigen for ELISA kits development,monkey herpes B virus gG gene sequences is firstly down-load from Genbank database.Online analysis system IEDB and SOSUI are then adopted for the gene sequence bioinformatic analysis of the coding region.The prediction is carried out on protein encoded gG gene physico-chemical properties,solubility and surface accessibility,transmembrane region epitopes and linear B -cell epitopes.The results show that membrane protein gG protein herpes B virus is a soluble protein,having a trans-membrane helix,and relatively richβ-corners,whose protein sequence has a plurality of antigenic epitopes and dominant B-cell linear epitopes.It has the potential to serve as a monkey B virus vaccine candidate antigen and antibody diagnostic antigen.