CAJ | 학술논문

从健康茶树体内分离出53株耐铝内生细菌，通过促生指标评测，筛选出一株具有显著促生功能的内生细菌G3。该菌株具有明显的固氮与溶磷作用，可以产生植物生长素及铁载体，并具有1-氨基环丙烷-1-羧酸脱氨酶活性，同时该菌株还对小麦种子萌发及根芽生长具有显著的促进作用。经细菌16S rDNA序列比对确定G3属于伯克霍尔德菌属，并与Burkholderia cepacia具有99％的相似度。铝离子吸附实验则表明，在pH 5．0，温度30℃时，G3对铝离子的吸附效果最好，吸附平衡时间为1 h。
종건강다수체내분리출53주내려내생세균，통과촉생지표평측，사선출일주구유현저촉생공능적내생세균G3。해균주구유명현적고담여용린작용，가이산생식물생장소급철재체，병구유1-안기배병완-1-최산탈안매활성，동시해균주환대소맥충자맹발급근아생장구유현저적촉진작용。경세균16S rDNA서렬비대학정G3속우백극곽이덕균속，병여Burkholderia cepacia구유99％적상사도。려리자흡부실험칙표명，재pH 5．0，온도30℃시，G3대려리자적흡부효과최호，흡부평형시간위1 h。
Fifty three aluminum-resistant endophytic bacteria were isolated from the healthy tissues of a tea tree.Among those strains,the G3 strain was selected for further studding based on the evaluation of its growth-promoting capabilities .This strain exhibited remarkable plant-growth-promoting properties including phosphate solubilization activity ,production of phytohormone ,nitrogen fixation ,siderophore biosynthesis and 1-aminocyclopropane-1-carboxylate deaminase activity .Moreover ,the G3 strain was also proved to facilitate seed germination and seedling growth of wheat .The 16S rDNA sequence analysis suggested that the G 3 strain was a species of Burkholderia sharing a sequence similarity of 99%with Burkholderia cepacia.In addition ,the experi-ment results of aluminum ion adsorption showed that the G 3 strain got its optimum adsorption activity at pH 5.0 and 30 ℃and the adsorption equilibrium time was about 1 hour .