CAJ | 학술논문

为培育芳香繁密的茶花新品种，研究以具有芳香、开花繁密特性的山茶属（ Camellia）连蕊茶组（ Sect． Theopsis）、植物为主要亲本，分别与金花茶组（ Sect．Chrysantha）、古茶组（ Sect．Archecamellia）和红山茶组（ Sect． Camellia）的部分种及品种进行远缘杂交试验；并通过荧光显微技术观察杂交授粉后花粉管行为。结果表明：以连蕊茶组（Sect．Theopsis）为母本的杂交组合，其座果率均为0，表现出严重不亲和性。荧光观察显示花粉管萌发后伸长缓慢且难以定向生长，并出现花粉管扭曲或先端膨大、胼胝质堆积、呈“S”型回折或节状生长现象；杂交组合毛花连蕊茶（ C．fraterna）×金花茶（ C．Nitidissma）的花粉管生长情况好于毛花连蕊茶（ C．fraterna）×越南抱茎茶（ C．amplexicaulis）、毛花连蕊茶（ C．fraterna）×杜鹃红山茶（ C．azalea）组合。
위배육방향번밀적다화신품충，연구이구유방향、개화번밀특성적산다속（ Camellia）련예다조（ Sect． Theopsis）、식물위주요친본，분별여금화다조（ Sect．Chrysantha）、고다조（ Sect．Archecamellia）화홍산다조（ Sect． Camellia）적부분충급품충진행원연잡교시험；병통과형광현미기술관찰잡교수분후화분관행위。결과표명：이련예다조（Sect．Theopsis）위모본적잡교조합，기좌과솔균위0，표현출엄중불친화성。형광관찰현시화분관맹발후신장완만차난이정향생장，병출현화분관뉴곡혹선단팽대、변지질퇴적、정“S”형회절혹절상생장현상；잡교조합모화련예다（ C．fraterna）×금화다（ C．Nitidissma）적화분관생장정황호우모화련예다（ C．fraterna）×월남포경다（ C．amplexicaulis）、모화련예다（ C．fraterna）×두견홍산다（ C．azalea）조합。
In order to produce new aromatic cultivars with bloom brilliance ,distant crossbreeding was con-ducted by using species of Sect .Theopsis as female parents and C.Nitidissma( Sect.Chrysantha),C.amplexi-caulis(Sect.ArcheCamellia),original species and cultivars of Sect .Camellia as male parents.Fluorescent mi-croscopy was used to observe pollen tubes ’ behavior .The results showed that 0%fruit-set rate appeared in all crosses with species of Sect .Theopsis as female parents.Pollen germinated slowly and grew horizontally ’not di-rected towards ovary , displaying a series of abnormal behavior including distorted or apex inflated , folded or twisty pollen tubes ,‘S’ shape or nodular growth ,and lots of callose depositiing .The pollen tube grew better in the cross C.fraterna×C.Nitidissma than in the other two crosses .