江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
2期
300-304
,共5页
李贤超%吴友根%杨东梅%张军锋
李賢超%吳友根%楊東梅%張軍鋒
리현초%오우근%양동매%장군봉
广藿香%盐胁迫%叶肉细胞%超微结构
廣藿香%鹽脅迫%葉肉細胞%超微結構
엄곽향%염협박%협육세포%초미결구
Pogostemon cablin%salt stress%mesophyll cells%ultrastructure
对海南广藿香盐胁迫下超微结构的变化进行研究分析。结果表明,未经NaCl胁迫的叶片叶绿体结构紧实,膜系统完整;低盐浓度下(40 mmol/L NaCl)叶绿体内存在较多较大的淀粉粒,并被大量的线粒体包围;高盐浓度下(200 mmol/L NaCl)淀粉粒减少,波浪状的细胞质膜从细胞壁缩回,叶绿体被膜严重损伤而降解,边缘结构遭到破坏,类囊体膨大,片层结构排列松散且片层间距加大,叶绿体降解呈离散状态,线粒体嵴膨大,部分出现半空泡化现象。这表明盐胁迫对苗期广藿香的生长具有抑制作用,盐浓度愈高,抑制效果愈明显,这为广藿香耐盐品种选育和发展广藿香在盐碱地种植提供细胞学依据。
對海南廣藿香鹽脅迫下超微結構的變化進行研究分析。結果錶明,未經NaCl脅迫的葉片葉綠體結構緊實,膜繫統完整;低鹽濃度下(40 mmol/L NaCl)葉綠體內存在較多較大的澱粉粒,併被大量的線粒體包圍;高鹽濃度下(200 mmol/L NaCl)澱粉粒減少,波浪狀的細胞質膜從細胞壁縮迴,葉綠體被膜嚴重損傷而降解,邊緣結構遭到破壞,類囊體膨大,片層結構排列鬆散且片層間距加大,葉綠體降解呈離散狀態,線粒體嵴膨大,部分齣現半空泡化現象。這錶明鹽脅迫對苗期廣藿香的生長具有抑製作用,鹽濃度愈高,抑製效果愈明顯,這為廣藿香耐鹽品種選育和髮展廣藿香在鹽堿地種植提供細胞學依據。
대해남엄곽향염협박하초미결구적변화진행연구분석。결과표명,미경NaCl협박적협편협록체결구긴실,막계통완정;저염농도하(40 mmol/L NaCl)협록체내존재교다교대적정분립,병피대량적선립체포위;고염농도하(200 mmol/L NaCl)정분립감소,파랑상적세포질막종세포벽축회,협록체피막엄중손상이강해,변연결구조도파배,류낭체팽대,편층결구배렬송산차편층간거가대,협록체강해정리산상태,선립체척팽대,부분출현반공포화현상。저표명염협박대묘기엄곽향적생장구유억제작용,염농도유고,억제효과유명현,저위엄곽향내염품충선육화발전엄곽향재염감지충식제공세포학의거。
The ultrastructure changes of P.cablin stressed with different concentrations of salinity were stud-ied using tranmission electron microscope .The results indicated ,the regular and compact chloroplast had integrat-ed membrane system under the water free of salinity .The chloroplast abundant with big starch grain was closed in a mass of mitochondria under low concentration of salinity ( 40 mmol/L NaCl ) .The membrane system of chloro-plast departing from cell wall and less of starch grain was degenerated and destroyed ,the thylakoids of dissocia-tive chloroplast was intumescent ,degenerated and loosened obviously ,and the half-vacuolated mitochondria were observed under higher concentration of salinity .This suggested that salt stress had inhibitory effects on the growth of P.cablin seedling.The higher the salt concentration was ,more obvious the inhibition effect was .It would pro-vide the cytological basis for salt-tolerant variety breeding and patchouli planting in saline -alkali land .