江西林业科技
江西林業科技
강서임업과기
JIANGXI FORESTRY SCIENCE AND TECHNOLOGY
2014年
2期
14-17
,共4页
刘跃钧%陈世通%王立平%傅冰
劉躍鈞%陳世通%王立平%傅冰
류약균%진세통%왕립평%부빙
方竹%合江方竹%分子标记%遗传多样性%研究
方竹%閤江方竹%分子標記%遺傳多樣性%研究
방죽%합강방죽%분자표기%유전다양성%연구
Chimonobambusa quadrangularis%Ch. hejiangensis%molecular markers%genetic diversity%research
目的：，建立及优化方竹和合江方竹的ISSR-PCR反应体系，分析其12个种质资源的遗传多样性。方法，采用L16（45）正交试验建立、优化ISSR-PCR反应体系，利用该体系分析12个不同种源的方竹和合江方竹的遗传多样性。结果最佳ISSR-PCR的最佳体系为：1×Reaction Buffer、dNTPs 100μmol/L、MgCl22.5 mmol/L、引物0.1μmol/L、Taq 0.8 U以及DNA 20 ng。12个供试竹种被分为两大类群，其中除方竹遂昌种源2外的其他方竹竹种亲缘关系相当接近，归为一个亚群；合江方竹杭州种源和合江方竹莲都种源归到一个小亚群中。
目的：，建立及優化方竹和閤江方竹的ISSR-PCR反應體繫，分析其12箇種質資源的遺傳多樣性。方法，採用L16（45）正交試驗建立、優化ISSR-PCR反應體繫，利用該體繫分析12箇不同種源的方竹和閤江方竹的遺傳多樣性。結果最佳ISSR-PCR的最佳體繫為：1×Reaction Buffer、dNTPs 100μmol/L、MgCl22.5 mmol/L、引物0.1μmol/L、Taq 0.8 U以及DNA 20 ng。12箇供試竹種被分為兩大類群，其中除方竹遂昌種源2外的其他方竹竹種親緣關繫相噹接近，歸為一箇亞群；閤江方竹杭州種源和閤江方竹蓮都種源歸到一箇小亞群中。
목적：，건립급우화방죽화합강방죽적ISSR-PCR반응체계，분석기12개충질자원적유전다양성。방법，채용L16（45）정교시험건립、우화ISSR-PCR반응체계，이용해체계분석12개불동충원적방죽화합강방죽적유전다양성。결과최가ISSR-PCR적최가체계위：1×Reaction Buffer、dNTPs 100μmol/L、MgCl22.5 mmol/L、인물0.1μmol/L、Taq 0.8 U이급DNA 20 ng。12개공시죽충피분위량대류군，기중제방죽수창충원2외적기타방죽죽충친연관계상당접근，귀위일개아군；합강방죽항주충원화합강방죽련도충원귀도일개소아군중。
The purpose of the establishment and optimization of ISSR -PCR reaction system of Chimonobambusa quadrangularis and Ch. hejiangensis was to analyze the genetic diversity of its 12 germplasm. Method using L16 (45) orthogonal test build, optimize ISSR-PCR reaction system, the genetic diversity of 12 different provenances from Ch. quadrangularis and Ch. hejiangensis was analyzed by the system. Results showed that: The best system for optimal ISSR-PCR: 1 × Reaction Buffer, dNTPs 100μmol/L, MgCl2 2.5 mmol/L, primer 0.1μmol/L, Taq 0.8 U and DNA 20 ng. 12 bamboo species tested were divided into two groups, the kinship of Ch. Quadrangularis' provenances was related close, except the Suichang provenance No. 2, which classified as a subgroup;The Hangzhou provenance and Liandu provenance of Ch. hejiangensis were normalized to a small subgroup.
