CAJ | 학술논문

【目的】研究碱法提取方格星虫体壁中水溶性方格星虫多糖的条件及优化。【方法】根据料液比、浸提时间、浸提温度、碱液浓度对多糖得率的影响，并通过正交试验得到方格星虫水溶性多糖浸提工艺优选因素组合。【结果】影响方格星虫多糖得率的因素主次顺序为浸提温度＞料液比＞浸提时间＞碱液浓度；最佳浸提条件为温度50℃，料液比1∶6 g/mL，时间4h，碱液（NaOH）的质量分数为8％。【结论】最佳浸提工艺条件下多糖浸提提取率最大为1．81％。
【목적】연구감법제취방격성충체벽중수용성방격성충다당적조건급우화。【방법】근거료액비、침제시간、침제온도、감액농도대다당득솔적영향，병통과정교시험득도방격성충수용성다당침제공예우선인소조합。【결과】영향방격성충다당득솔적인소주차순서위침제온도＞료액비＞침제시간＞감액농도；최가침제조건위온도50℃，료액비1∶6 g/mL，시간4h，감액（NaOH）적질량분수위8％。【결론】최가침제공예조건하다당침제제취솔최대위1．81％。
[Objective]To choose the optimum alkaline extraction process of water-soluble poly-saccharide from Sipunculus nudus Linnaeus.[Methods]The orthogonal test design with four factors was used to study the effect of the ratio of material to fluid,extraction time,extraction temperature and alkali concentration on the extracting rate of water-soluble polysaccharides from S.nudus .[Results]The order of the factors that affect the yield ofS.nudus polysaccharide is extraction temperature>ratio of material to fluid> extraction time> alkali concentration;the optimum extraction conditions were temperature 50℃,ratio of material to fluid 1 ∶ 6 g /mL,time 4h and alkali (NaOH)content of 8%.[Conclusion]Under the above conditions,the optimum extraction rate is 1.81%.