CAJ | 학술논문

【目的】从动物粪便样品中分离能利用合成气生产乙醇的菌株，并对其进行鉴定及初步发酵实验，为工业利用合成气生产乙醇奠定理论基础。【方法】利用富集驯化培养技术，以合成气为唯一碳源，分离筛选合成气利用菌，通过形态观察、生理生化实验及16S rRNA 序列分析鉴定菌株，并利用菌株进行初步发酵实验。【结果】分离到1株可以发酵合成气生产乙醇的菌株，分子鉴定结果表明，该菌株为 Clostridium ljungdahlii 。生理生化分析结果显示：该菌是严格厌氧型革兰氏阳性菌，短杆状，有运动性，芽孢很少见，生长必须因子包括酵母粉和维生素 B 族；最适碳源为果糖，最适生长温度35～37℃，最适 pH 值为6．0～7．0。合成气发酵实验结果显示：37℃，厌氧静止发酵30d，乙醇最高产量达到2．58g/L，添加 BESA、莫能菌素、丁基橡胶颗粒可以使乙醇产量分别提高49％、70％、4．3％，最大产量达到4．31g/L。【结论】从动物粪便中分离到1株 Clostridium ljungdahlii ，该菌能够利用合成气生产乙醇，为进一步研究和开发新型生物质材料提供了基础资料。
【목적】종동물분편양품중분리능이용합성기생산을순적균주，병대기진행감정급초보발효실험，위공업이용합성기생산을순전정이론기출。【방법】이용부집순화배양기술，이합성기위유일탄원，분리사선합성기이용균，통과형태관찰、생리생화실험급16S rRNA 서렬분석감정균주，병이용균주진행초보발효실험。【결과】분리도1주가이발효합성기생산을순적균주，분자감정결과표명，해균주위 Clostridium ljungdahlii 。생리생화분석결과현시：해균시엄격염양형혁란씨양성균，단간상，유운동성，아포흔소견，생장필수인자포괄효모분화유생소 B 족；최괄탄원위과당，최괄생장온도35～37℃，최괄 pH 치위6．0～7．0。합성기발효실험결과현시：37℃，염양정지발효30d，을순최고산량체도2．58g/L，첨가 BESA、막능균소、정기상효과립가이사을순산량분별제고49％、70％、4．3％，최대산량체도4．31g/L。【결론】종동물분편중분리도1주 Clostridium ljungdahlii ，해균능구이용합성기생산을순，위진일보연구화개발신형생물질재료제공료기출자료。
[Objective]To isolate and identify a microbial strain capable of producing ethanol from syngas fermentation from animal waste.[Methods]The enrichment culture using syngas as the sole carbon source was utilized to isolate the strain,which was identified according to the ob-servation of morphology,physiological and biochemical tests,and analysis of 16S rRNA gene sequences.The isolated strain was utilized for preliminary fermentation.[Results]A strain ca-pable of fermenting syngas into ethanol was isolated successfully.The strain was identified as Clostridium ljungdahlii ,which is a strict anaerobe and gram-positive.The cells are rod-shaped and show motility;spores are formed in-frequently;the strain requires a minimum level of yeast extract (0.1%) and B-vitamins for growth;substrate optimum is fructose;temper-ature optimum range is 35 ~37℃;pH optimum range is 6.0 ~ 7.0;batch fermentation reveals that under anaerobic conditions the highest yield of ethanol in serum bottles at 37℃ was 2.58g/L after 30d;by addition of BESA,monensin and butyl rubber particles yields of ethanol can in-crease 49%,70% and 4.3%,respectively,with the highest yield of ethanol at 4.31g/L.[Con-clusion]Clostridium ljungdahlii was capable of producing ethanol from syngas fermentation.