大连海洋大学学报
大連海洋大學學報
대련해양대학학보
JOURNAL OF DALIAN FISHERIES UNIVERSITY
2014年
2期
161-166
,共6页
黄婷%李莉萍%王瑞%张彬%甘西%罗洪林%陈福艳%蔡小辉%杨传萍%梁万文%陈明
黃婷%李莉萍%王瑞%張彬%甘西%囉洪林%陳福豔%蔡小輝%楊傳萍%樑萬文%陳明
황정%리리평%왕서%장빈%감서%라홍림%진복염%채소휘%양전평%량만문%진명
卵形鲳鲹%无乳链球菌%海豚链球菌%16S rRNA%药敏试验
卵形鯧鲹%無乳鏈毬菌%海豚鏈毬菌%16S rRNA%藥敏試驗
란형창소%무유련구균%해돈련구균%16S rRNA%약민시험
Trachinotus ovatus%Streptococcus agalactiae%S. iniae%16S rRNA%drug sensitivity test
于2011年8月对广西北海疑似链球菌感染的卵形鲳鲹Trachinotus ovatus进行病原分离鉴定，经人工感染试验，确定分离的两株病原菌(编号为TSG002和TSG004)为卵形鲳鲹的致病菌，然后对两株病原菌进行形态学、生理生化特征、16S rRNA基因序列和二重PCR快速检测综合鉴定，构建系统进化树，最后对两株菌进行药敏分析。根据染色形态特征和二重PCR快速检测，初步鉴定菌株TSG002和TSG004分别为无乳链球菌和海豚链球菌，菌株TSG002和TSG004的16S rRNA基因序列(登录号分别为KF826095和KF826094)分别与无乳链球菌 ATCC13813 strain JCM 5671基因(登录号 NR040821.1)和海豚链球菌ATCC29178基因(登录号AF335572.1)的相似性最高，均达99%。药敏试验结果表明，两株菌对头孢曲松、头孢呋辛、头孢哌酮、恩诺沙星、氧氟沙星、阿莫西林、多粘菌素B、头孢噻吩和头孢他啶均敏感。研究表明，感染无乳链球菌和海豚链球菌的卵形鲳鲹可发病死亡，在病鱼中同时分离到两种链球菌尚属首例。
于2011年8月對廣西北海疑似鏈毬菌感染的卵形鯧鲹Trachinotus ovatus進行病原分離鑒定，經人工感染試驗，確定分離的兩株病原菌(編號為TSG002和TSG004)為卵形鯧鲹的緻病菌，然後對兩株病原菌進行形態學、生理生化特徵、16S rRNA基因序列和二重PCR快速檢測綜閤鑒定，構建繫統進化樹，最後對兩株菌進行藥敏分析。根據染色形態特徵和二重PCR快速檢測，初步鑒定菌株TSG002和TSG004分彆為無乳鏈毬菌和海豚鏈毬菌，菌株TSG002和TSG004的16S rRNA基因序列(登錄號分彆為KF826095和KF826094)分彆與無乳鏈毬菌 ATCC13813 strain JCM 5671基因(登錄號 NR040821.1)和海豚鏈毬菌ATCC29178基因(登錄號AF335572.1)的相似性最高，均達99%。藥敏試驗結果錶明，兩株菌對頭孢麯鬆、頭孢呋辛、頭孢哌酮、恩諾沙星、氧氟沙星、阿莫西林、多粘菌素B、頭孢噻吩和頭孢他啶均敏感。研究錶明，感染無乳鏈毬菌和海豚鏈毬菌的卵形鯧鲹可髮病死亡，在病魚中同時分離到兩種鏈毬菌尚屬首例。
우2011년8월대엄서북해의사련구균감염적란형창소Trachinotus ovatus진행병원분리감정，경인공감염시험，학정분리적량주병원균(편호위TSG002화TSG004)위란형창소적치병균，연후대량주병원균진행형태학、생리생화특정、16S rRNA기인서렬화이중PCR쾌속검측종합감정，구건계통진화수，최후대량주균진행약민분석。근거염색형태특정화이중PCR쾌속검측，초보감정균주TSG002화TSG004분별위무유련구균화해돈련구균，균주TSG002화TSG004적16S rRNA기인서렬(등록호분별위KF826095화KF826094)분별여무유련구균 ATCC13813 strain JCM 5671기인(등록호 NR040821.1)화해돈련구균ATCC29178기인(등록호AF335572.1)적상사성최고，균체99%。약민시험결과표명，량주균대두포곡송、두포부신、두포고동、은낙사성、양불사성、아막서림、다점균소B、두포새분화두포타정균민감。연구표명，감염무유련구균화해돈련구균적란형창소가발병사망，재병어중동시분리도량충련구균상속수례。
Two bacterial strains TSG002 and TSG004 were isolated from the diseased ovate pompano Trachinotus ovatus collected in Beihai,Guangxi Province,China in August of 2011,and were confirmed to be the pathogens of the diseased ovate pompano by animal challenge test, and by morphology, physiological and biochemical examina-tion and 16S rRNA genetic analysis. In addition,the sensitivity of both strains to twenty-two antibiotics were stud-ied. The results showed that strains TSG002 and TSG004 were similar to bacteria Streptococcus agalactiae and S. in-iae, based on the morphology, physiology and biochemistry examinations. The phylogenetic analysis revealed that the l6 S rRNA sequence of strains TSG002 ( KF826095 ) and TSG004 ( KF826094 ) has high similarity ( 99%) to S. agalactiae (NR040821. 1) and S. iniae (AF335572. 1) in GenBank. Phylogenetic tree of Streptococcus spp. built according to 16S rRNA genes also showed that strains TSG002 and TSG004 are classified into S. agalactiae and S. iniae group, respectively. Both strains were found to be highly sensitive to nine drugs, including ceftriaxone, enrofloxacin, ofloxacin, amoxicillin and cephalothin. It is concluded that ovate pompano can be infected by S. aga-lactiae and S. iniae, which were firstly and simultaneously found in one diseased ovate pompano.
