CAJ | 학술논문

对杂交三倍体泥鳅Misgurnus anguillicaudatus (2n意×4n裔, DT)和二倍体泥鳅(2n意×2n裔, DD)的胚胎发育进行观察,详细记录了其胚胎发育的形态特征和发育特点。结果表明： DT受精卵在水温(25±1)℃的条件下,整个胚胎发育过程约需36 h 19 min；对照组DD受精卵在水温(25±1)℃的条件下,整个胚胎发育过程约需35 h 57 min； DT ( DD)的胚胎发育包括卵裂期、囊胚期、原肠期、神经胚期、器官形成期和孵化出膜期6个时期, DT ( DD )受精卵约40 min (40 min )后进入卵裂期,3 h 45 min (3 h 40 min)后进入囊胚期,6 h 30 min (6 h 24 min)后进入原肠期,9 h 32 min (9 h 20 min)后进入神经胚期,15 h 45 min (15 h 30 min)后进入器官形成期,36 h 19 min (35 h 57 min)后出膜。研究表明, DT的胚胎发育时序及形态特征与DD基本相似,说明DT具有正常的胚胎发育过程,本研究可为杂交三倍体泥鳅苗种培育提供生物学±据。
대잡교삼배체니추Misgurnus anguillicaudatus (2n의×4n예, DT)화이배체니추(2n의×2n예, DD)적배태발육진행관찰,상세기록료기배태발육적형태특정화발육특점。결과표명： DT수정란재수온(25±1)℃적조건하,정개배태발육과정약수36 h 19 min；대조조DD수정란재수온(25±1)℃적조건하,정개배태발육과정약수35 h 57 min； DT ( DD)적배태발육포괄란렬기、낭배기、원장기、신경배기、기관형성기화부화출막기6개시기, DT ( DD )수정란약40 min (40 min )후진입란렬기,3 h 45 min (3 h 40 min)후진입낭배기,6 h 30 min (6 h 24 min)후진입원장기,9 h 32 min (9 h 20 min)후진입신경배기,15 h 45 min (15 h 30 min)후진입기관형성기,36 h 19 min (35 h 57 min)후출막。연구표명, DT적배태발육시서급형태특정여DD기본상사,설명DT구유정상적배태발육과정,본연구가위잡교삼배체니추묘충배육제공생물학±거。
The embryonic development was morphologically observed in hybrid triploid(DT,2n♀×4n♂) and dip-loid loach (DD,2n♀×2n♂) Misgurnus anguillicaudatus. The embryonic development in DD took 35 h 57 min at water temperature of(25±1)℃, while the embryonic development in DT was completed in 36 h 19 min at the same conditions. The embryonic development included six phases cleavage, blastula, gastrula, neurula, organogenesis, and hatching stages. Fertilized eggs in DT( DD) developed into cleavage stage in 40 min (40 min) after fertiliza-tion, blastula stage in 3 h 45 min(3 h 40 min) after fertilization, gastrula stage in 6 h 30 min(6 h 24 min) after fertilization, neurula stage in 9 h 32 min(9 h 20 min) after fertilization, organogenesis stage in 15 h 45 min(15 h 30 min) after fertilization, and hatching in 36 h 19 min(35 h 57 min) after fertilization. The sequence of embryon-ic development in DT was similar to that in DD, though blastula stage in DT was observed later than that in DD. The findings indicate that the hybrid triploid loach have normal embryonic development, providing biological evi-dences of embryonic development for breeding of triploid loach in aquaculture.