现代仪器与医疗
現代儀器與醫療
현대의기여의료
Modern Instrument and Medical Treatment
2014年
3期
72-74
,共3页
陈丽芳%杨建云%肖炳坤%黄荣清
陳麗芳%楊建雲%肖炳坤%黃榮清
진려방%양건운%초병곤%황영청
反相高效液相色谱法%阿魏酸衍生物%含量测定%有关物质
反相高效液相色譜法%阿魏痠衍生物%含量測定%有關物質
반상고효액상색보법%아위산연생물%함량측정%유관물질
RP-HPLC%ferulic acid derivative%assay%related substances
目的：建立测定阿魏酸衍生物（BL）含量及其有关物质的高效液相色谱法。方法：采用Agilent Eclipse C18(250mm×4.6mm,5μm)色谱柱，流动相为甲醇-0.1%乙酸（42：58），流速1.0 mL·min-1；检测波长228nm；进样量20μL；柱温：40℃。结果：在该条件下BL主峰与有关物质得到很好的分离；检测浓度在0.08～0.32mg·mL-1范围内具有良好的线性关系（r=0.9996）；检测限和定量限分别为8n g和36n g。结论：该方法准确可靠，灵敏度高，专属性好，可用于BL含量及其有关物质的测定。
目的：建立測定阿魏痠衍生物（BL）含量及其有關物質的高效液相色譜法。方法：採用Agilent Eclipse C18(250mm×4.6mm,5μm)色譜柱，流動相為甲醇-0.1%乙痠（42：58），流速1.0 mL·min-1；檢測波長228nm；進樣量20μL；柱溫：40℃。結果：在該條件下BL主峰與有關物質得到很好的分離；檢測濃度在0.08～0.32mg·mL-1範圍內具有良好的線性關繫（r=0.9996）；檢測限和定量限分彆為8n g和36n g。結論：該方法準確可靠，靈敏度高，專屬性好，可用于BL含量及其有關物質的測定。
목적：건립측정아위산연생물（BL）함량급기유관물질적고효액상색보법。방법：채용Agilent Eclipse C18(250mm×4.6mm,5μm)색보주，류동상위갑순-0.1%을산（42：58），류속1.0 mL·min-1；검측파장228nm；진양량20μL；주온：40℃。결과：재해조건하BL주봉여유관물질득도흔호적분리；검측농도재0.08～0.32mg·mL-1범위내구유량호적선성관계（r=0.9996）；검측한화정량한분별위8n g화36n g。결론：해방법준학가고，령민도고，전속성호，가용우BL함량급기유관물질적측정。
Objective: To establish an RP-HPLC method for determination of the content of ferulic acid derivative (BL) and its related substances.Methods: Agilent Eclipse C18(250mm×4.6mm,5μm) column was adopted;the mobile phase consisted of methanol-0.1% acetic acid (42:58) at the lfow rate of 1.0 mL·min-1;The ultraviolet detection wavelength was set at 228nm;Loaded volume was 20μL;the column temperature was 40℃.Results: Using this method,BL and its related substances were well separated;The standard curve was rectilinear in the range of 0.08mg·mL-1~0.32 mg·mL-1 (r=0.9996);The limit of detection and quantiifcation were 8ng and 36ng.Conclusion: The method appeared to be accurate, reliable, sensitive, speciifc and can be used for the determination of the content BL and its related substances.
